抗体芯片分析炎性细胞因子在严重脓毒症中的表达.pdfVIP

. 830 • ·临床研究· 抗体芯片分析炎性细胞因子在严重服毒症巾 的表达 严静张召才罄罔龙虞意华胡才觉吴先 {摘要1 日的 应用抗体芯片分析严重服毒瘾患者血清中炎性细胞因子的表达。方法 12 例符合严囊 版毒症诊断标准的患者和 10 例年龄、性别匹配的对照组患者入选。用生物萦标记从两组患者血清中提取的 总蛋白，然后与预先点有 40 个主要炎症相关细胞回子的特异抗体的抗体芯片膜反应，目标蛋白与辣根过氧 化物酶(日附)标记的抗链草草生物素抗体结合并曝光显示反应信号，最后用激光扫描仪将其转化为图像文 件进行分析。结果 与对照组相比，除毒症患者组血清中多种炎性细胞因子包括促炎因子、抗炎因子、趋 化因子、某些细胞因子受体等的表达水平大幅度提高 但抗炎因子白介素(IL) 2 ，皿 4、 -13、四 15 表 达明显下降。结论 严囊除毒症病程中存在炎症反应过度、抗炎因子与倪炎因子失衡。 {关键词}抗体芯片;服毒症;细胞回子;炎疲;蛋白芯片 Role of anti胁dy chin in 翩翩lysis of inf1ammaωry cytokine exprωsion in severe sep哩:is YAN Jìng , ZHANG Z.归。cai ， CAI Gω1m略， YU Yihua , HU Cai迅ω， WU Liang. lntens时 Care U时， Zhejiang Ho机时， Ha略Zhou 310013 , China Corresponding author: ZHANG Zhaocai Email: zhaocai @ gmail. ωm [Abstractl Objective To investigate the expression of i aωn antibody chi协p. Methods Twelve pa创ti沁ent怡s wit血h 陇sev刊ere sepsis and other 10 patients whose 鸣喜a e and 阱gen时de町rw附ere matched w附ere enrolled in 趴thi怡8 自刷阳d句y. Proteins from patients were labeled with biotin. ηle biotin - labeled proteins reacted with antibody chips , on which there we陀 antibodies of 40 major inflammatory cytokines. The target proteins wereωnjugated with streptomycin antibody labeled by horseradish peroxida附(HRP) , and signals were imaged by laser scanner. ResuJts 1n comparison 创th control group , the serum levels of inflammatory c严okines including pro 叩 and anti - inflammatory cytokines , chemokines and certain cytokines receptors were notably increased , whíle expression of anti 呵 inflammatory interleukin (IL) - 2 , - 4 , - 13 , - 15 wωremarkably decreased in sepsis group. Conclωlons Exc创s inflanlffiatory response and imbalance of pro 响 and anti - inflammatory cytokines were presented in the course of severe sepsis. [Key words 1Antibody 盯hip; Sepsis; Cytokine; 1nflammationj Protein array 服