芒果多糖的纯化与光谱分析.pdfVIP

※基础研究 食品科学 2012, Vol. 33, No. 07 93 芒果多糖的纯化与光谱分析 1 2 3 4 孙延芳 ，梁宗锁 ，张 欣 ，Un ger FRAN K ( 1.辽宁工程技术大学理学院，辽宁阜新 12 3000 ；2 .西北农林科技大学生命科学学院，陕西杨凌 7 12 100 ； 3.西北农林科技大学食品科学与工程学院，陕西杨凌 7 12 100 ；4 .维也纳大学生物药理和药理技术实验室， 奥地利维也纳 A-1090) 摘 要：采用热水浸提、乙醇沉淀法得到芒果多糖粗品；采用苯酚- 硫酸法测其多糖含量为(46.3 ±1.75)mg/g 。DEAE-25 纤维素和Seph adex G-75 凝胶层析柱进一步分离纯化得到白色絮状多糖；高效阴离子交换色谱法分析其单糖组成。 结果表明：该多糖是由鼠李糖、半乳糖和葡萄糖等单糖组成，其含量分别为(6 .3 ±1.4 0 ) 、( 1 1 .9 ±1. 7 6 ) m g / g 和 (13.8 ±1.52)mg/g 。红外光谱分析表明具有明显的多糖特征吸收峰，该多糖结构主要为β- 糖苷键连接的吡喃型葡聚糖。 关键词：芒果；多糖；高效阴离子交换色谱；红外光谱 Purification and Spectroscopic Analysis of Polysaccharide from Mango 1 2 3 4 SUN Yan-fang ，LIANG Zong-suo ，ZHANG Xin ，Unger FRANK (1. College of Science, Liaoning Technical University, Fuxin 123000, China ；2. College of Life Science, Northwest AF University, Yangling 7 12 100, China ；3. College of Food Science and Engineering, Northwest AF University, Yangling 7 12 100, China ； 4. Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna A-1090, Austria) Abstract：Crude polysaccharide was extracted from Mexico mango using hot water extraction followed by ethanol precipitation. The polysaccharide concentration of crude extract was (46.3 ±1.75) mg/g as determined by phenol-sulfuric acid assay. After further purification by DEAE-52 and Sephadex G-75 column chromatography, a white flocculent polysaccharide was obtained. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was