人骨髓间充质干细胞缝隙连接通讯功能的研究_宋晋刚.pdfVIP

DO I 牶牨牥牣牨牰牥牨牰牤j牣牨牥牥牥牠牭牬牥牬牣牪牥牥牭牣牨牰牣牥牥牴 27 16 　　　　　　　 V o l. 27, N o. 16 1648 2005 8 　　ACTA　ACADEM IAE　M ED IC INAE　M IL ITAR IS　TERT IAE　　 A ug. 2005 文章编号:1000-5404(2005)16-1648-04 1 1 1 1 1 1 2 1 宋晋刚 ,许建中, 王序全,周　强 , 罗　飞 , 何清义, 孙　玮 　　(:, 2 , , 400038) 　　　:目的　 (hum an m esenchym a l stem ce lls, hM SCs) (gap junc- tional in te rce llu lar communication, G JIC)。方法　, (luo rescence redistribu tion a ter photobleaching, FRA P), 5, 6-CFDA hM SCs, , hM SCs G J IC 。结果　, hM SC s (35.26 ±0.76)%。结论　。 　　:;; 　　:R 322. 7 1;R329. 27　　　 :A Study on gap junctional intercellular communication of human mesenchymal stem cells 1 1 1 1 1 1 2 1 SONG Jin-gang , XU J ian-zhong , WANG X u-quan , ZHOU Q iang , LUO Fe i, HE Q ing-y i, SUN W ei (D epa rtm en t o O r thopaed ics, Sou thw est Ho sp ita,l 2 D epa rtm ent o Cente r Laborato ry, C ollege o H igh A ltitudeM ilita ry M ed ic ine, ThirdM ilitary M edica l U niversity, Chongqing 4000 38, Ch ina) 　　Abstract:O bjective　To de te rm ine the gap junc tiona l interce llu lar comm un ica tion (G JIC )o hum an m esenchym a l stem ce lls (hM SC s). M e thods　The th ird genera tion hum an m esenchym a l stem ce lls we re u sed to observe the struc ture o gap junction by transm ission electron m icroscope (TEM ). The m ethod o luo res- cence redistribution a te r pho tobleach ing (FRA P )w as u sed to eva luate the unc tions o GJ IC through a laser scanning con oca l m ircroscope. Resu lts　The cellular luo rescence cou ld transpo