胃癌术后复发转移与抑癌基因Syk启动子甲基化的研究.pdfVIP

ìIE9医至2型24旦旦旦旦主一主豆塑主些应i Med旦旦凹旦生August 2012 ， Vol47 且08 .659. ·论著· 胃癌术后复发转移与抑癌基因 Syk 启动子甲基化的研究* 洪华章周凯黄琪傅平袁喜红王俊李坚 [摘要]目的研究胃癌组织中 Syk 基因启动子甲基化与术后复发转移的关系。方法采用甲基化特异性 PCR 法检测胃 癌组织及癌旁正常胃组织的 Syk 基因启动子甲基化状态。结果在 70 例胃癌组织中，Syk 基因启动子甲基化发生率为 45.7% (32斤。) ;在癌旁正常组织发生率分别为 0(0170) ;在胃癌组织中，Syk 基因甲基化发生率明显高于癌旁正常组织(PO.OOI)。检测 到 Syk 基因启动子甲基化的 32 例病例中，有 21 例病例术后发生复发转移，发生率为 65.6%(21132) ; 在未检测到 Syk 基因启 动子甲基化的 38 例病例中，仅有 7 例病例术后发生复发转移，发生率为 18.4%(7/38)(P0.001)。结论 (l)Syk 基因启动子甲基 化与胃癌术后复发转移密切相关。(2) 胃癌组织中 Syk 基因启动子甲基化的检测有助于胃癌术后复发转移的早期诊断及预后 评估。 [关键词]胃癌;Syk 基因:甲基化;复发转移;甲基化特异性 PCR I 申图分类号1 R735.2 The study of relationship between metbylation of Syk gene in promoter region and postoperative recurrence , metastasis in gastric carcinoma HONG Hua-zhang ,ZHOU Kai ,HUANG Qi , et 01. Dep旷tment 0/ Cadre Surgery , The Jiang刘丹阳山ia1 Peo- ple s Hospital ,Nanchang 330ω6 ， Chinι [Abstract] Objective To research of the relationship between the aberrant methylation of Syk gene and recuπence and metastasis after resection of gastric cancer. Methods By using methylation -specific polymerase chain reaction technique (MSP) , methylation of Syk promoter region from gastric cancer patients (tumor tissues , adjacent normal tissues) were detected. Results The frequency of methylation of Syk promoter region was 45.7%(32/7 0)in gastric cancer.In contrast, the frequencies of methylation of Syk promoter region were 0(0170)in adjacent normal tissues. The rates of methylation of Syk promoter in gastric cancer w部 high­ er than that of adjacent normal tissues (PO.OOl ).The frequency of postoperative recuπence and metastasis in 32 cases with Syk promoter methylation was revealed in 34.4% (11132) and that in 38 cases with Syk promoter unmethylation was revealed in 18.4%