展青霉素产生菌拮抗放线菌的分离、筛选与初步鉴定.pdfVIP

农业机械学报 2008 年 3 月 第 39 卷第 3 期 展青霉素产生菌拮抗放线菌的分离、筛选与初步鉴定铃 朱从会师俊玲杨保伟令贞民王瑞4 i摘要】 毒素产生菌在苹果贮藏期间容易产生展青霉素，为此，从苹果园、猿猴桃园、菜园的土壤中分离出 154 株放线菌，从中筛选出对 3 株展青霉素产生菌有拮抗作用的放线菌4 株，复筛选得到抑菌效果最好的 1 株(C16) ， 对展青霉的抑菌直径达 2.33 cm。通过形态观察、生理生化特征等方面的检测，初步鉴定该菌株属于放线菌目 (Actinomycetales)第wl科小单胞菌科( Micromonosporaceae )第M属小多胞菌属(Micr。如， ，zys仰叫。， 关键词:苹果展青霉素拮抗放线茵筛选鉴定 中图分类号: Q939.92 文献标识码:A Isolation Screening and Identification of Antagonistic Actinomycetes Having Ability in Inhibiting Patulin-producing Penicillium Zhu Conghui Shi Junling - Yang Baowei Ling Zhenmin Wang Rui (Northwest AF University) Abstract In order to find oút actinomycetes having ability in patulin-producing penicillum. 154 strains of actinomycetes having different colony characteristics were isolated from soils of apple orchard , kiwifruit orchard and vegetable orchard. After screening , 4 strains were selected for further screening because they had high ability in inhibiting the growth of 3 strains of patulin-producing pecillium on the agar medium. Strain C16 showed the highest inhibiting ability by producing 2.33 cm inhibitory diameter in agar plate t臼ts. Based on the morphologic , physiological and biochemical characteristics , C16 was identified as the Order of Actinom)日tales. the Family of Micromonos如raceae ， and the Genus of Micro如lys户ra. Key words Apple. Patulin, Antagonistic actinomycetes , Screening , Identification 物间的拮抗作用抑制展青霉素产生菌的生长与产毒 引言 具有环境友好、没有农药残留等优点。放线菌广泛 存在于各种土壤中，能够产生多种抑菌活性物质，易 展青霉素(Patulin，简称 PAT) 是一种具有致 癌、致畸、致突变作用的神经性真菌毒素