葡萄球菌生物膜检测及与耐药性的关系.docVIP

葡萄球菌生物膜检测及与耐药性的关系 　　【摘要】 目的 探讨临床标本中葡萄球菌形成生物膜的状况，研究有无生物膜的葡萄球菌的耐药性差异,帮助临床根据药敏结果选用抗菌药物，及时有效控制葡萄球菌医院感染。 　　方法 采用刚果红,微量平板法检测生物膜形成,观察葡萄糖、乙醇、氯化钠对生物膜形成的影响;对临床标本分离的葡萄球菌进行鉴定和药敏试验。结果 刚果红法检测腐生葡萄球菌、金黄色葡萄球菌(金葡菌)?p表皮葡萄球菌生物膜阳性率分别为10.0%、16.7%、40.0%；生物膜半定量法：表皮葡萄球菌生物膜阳性率TSB、TSB-0.25%Glu、TSB-2%Alc、TSB-4%Alc、TSB-2%NaCl、TSB-4%NaCl分别为13.5%、34.6%、33.3%、29.2%、19.4%、32.1%，其中0.25%Glu、TSB-2%Alc、TSB-4%NaCl为最佳浓度；药敏结果，产生生物膜的葡萄球菌的耐药性对青霉素、红霉素和复方新诺明的耐药有增加趋势，耐药率分别为：86.43%、82.44%、78.35%。结论 临床标本葡萄球菌绝大部分能产生生物膜、葡萄糖、乙醇、氯化钠能促进生物膜形成；葡萄球菌对临床常用的抗菌药物耐药性严重，对其引起的感染可用万古霉素。 　　?ぁ竟丶?词】 葡萄球菌；生物膜；耐药性；药敏试验?? 　　文章编号：1003-1383(2008)03-0252-03 中图分类号：R 378.110.446.5文献标识码：A?お? 　　 　　Analysis staphylococcal biofilm and relationship to its drug resistanted 　　Zhang Li ping1,LI Qing2,Guna1,Li Ling ling2,Tian gang2?? 　　(1.Laboratory of First Hospital,Kashi Xinjiang 844000,China;?? 　　2.Laboratory of Xinjiang ChineseMedical Hospital，Xinjiang 830000,China?? 　　3.Laboratory of Gong An Hospital，Tianjin,300042,China)?? 　　【Abstract】 Objective To study the formation of staphylococcal biofilm and the difference of drug resistance between Staphylococcus with or without biofilm,helping to choose the antibiotic according to the drug sensitive trial,controlling the staphylococcal inffectin effectivly and timely. 　　Methods Biofilm-forming ability was determined by congo red agar (CAR) and a semi-quantitative microtiter assay using trypticase soy broth .Glucose,alcohol and Nacl were added to the medium separately to test their effect on biofilm formation by staphylococcal isolates.Results Biofilm formation was positive by 10.0% to Staphylococcus saprophyticus，16.7% to Staphylococcus Aureus, 40% to Staphylococcus epidermidis by congo red agar. The positive rate of biofilm-forming by Staphylococcus epidermidis in TSB, TSB-0.25% glucose, TSB-2% alcohol, TSB-4% alcohol, TSB-2% NaCl and TSB-4% NaCl were 13.5%、34.6%、33.3%、29.2%、19.4% and 32.1%respectively. drug sensitivity results shown that more and moreStaphylococcus with biofilm had drug