【3.5】2013年隐球菌脑膜炎临床及微生物情况.pdfVIP

Revista da Sociedade Brasileira de Medicina Tropical 46(3):343-347, May-Jun, 2013 /10.1590/0037-8682-0061-2012 MCaseajor ReportArticle Clinical and microbiological features of cryptococcal meningitis Lúcia Kioko Hasimoto e Souza[1], Carolina Rodrigues Costa[1], Orionalda de Fátima Lisboa Fernandes[1], Fernando Yano Abr?o[1], Thaisa Cristina Silva[1], Carolina Martins Treméa[1] and Maria do Rosário Rodrigues Silva[1] [1]. Laboratório de Micologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goias, Goiania, GO. ABSTRACT Introduction: In this study, the clinical features, underlying diseases and clinical outcomes of patients with cryptococcosis were investigated. In addition, a molecular analysis of the Cryptococcus neoformans species complex isolated from these patients was performed. Methods: A prospective study of 62 cases of patients with cryptococcal infection was conducted at the Hospital de Doen?as Tropicais de Goiás Dr. Anuar Auad from 2009-2010. Cryptococcal meningitis cases were diagnosed by direct examination and cerebrospinal ? uid (CSF) sample culture. The pro? ling of these patients was assessed. The CSF samples were submitted to India ink preparation and cultured on Sabouraud dextrose agar, and C. neoformans was identi? ed by the production of urease, a positive phenoloxidase test and assimilation of carbohydrates. C. neoformans and C. gattii isolates were distinguished by growth on L-canavanine-glycine-bromothymol blue medium, and molecular analysis was conducted via PCR ? ngerprinting reactions using M13 and (GACA)4 primers. Results: From the 62 patients with cryptococcosis, 71 isolates of CSF were obtained; 67 (94.4%) isolates were identi? ed as C. neoformans var. grubii/