聚氧化乙烯改善大鼠慢性缺血后肢的血液灌注.PDFVIP

J South Med Univ, 2017, 37(1): 113-118 doi 10.3969/j.issn. 1673-4254.2017.01.21 ·113 · 基础研究 聚氧化乙聚氧化乙烯改善大鼠慢性缺血后肢的血液灌注烯改善大鼠慢性缺血后肢的血液灌注 1 1 2 郑德仲 ，周 滔 ，查道刚 1 2 南方医科大学第三附属医院，广东 广州 510630；南方医科大学南方医院，广东 广州 510515 摘要：目的通过声学造影评价聚氧化乙烯（PEO）改善大鼠慢性缺血后肢的血液灌注的效果。方法 12只Wistar 大鼠通过结扎 一侧股动脉建立慢性后肢缺血模型，然后将大鼠随机分为实验组及对照组（n=6），隔天分别经颈内静脉输注PEO 和生理盐水，共 治疗2 周。在不同的时间点行声学造影检查测定大鼠缺血后肢骨骼肌的血流量并在第28 天测量血流储备量。结果 从第7 天 开始PEO 组大鼠缺血后肢骨骼肌的血流量高于对照组（P0.05），第28 天时PEO 组缺血后肢骨骼肌血流储备量及血管容积均显 著高于对照组（P0.05）。结论 PEO 显著提高了慢性后肢缺血模型大鼠骨骼肌的血流储备量和血流量，增加慢性缺血后肢骨骼 肌的血管容积，提示PEO 可能通过增加血流剪切力促进血管新生及动脉形成过程，从而改善缺血后肢的血液灌注。 关键词：聚氧化乙烯；动脉形成；血管新生；声学造影 Effects of polyethylene oxide on blood perfusion in the hind limbs of rats with chronic hindlimb ischemia 1 1 2 ZHENG Dezhong , ZHOU Tao , ZHA Daogang 1Department of Cardiology, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China; 2Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To evaluate the effects of polyethylene oxide (PEO) on blood perfusion in hind limb skeletal muscles in a rat model of chronic hind limb ischemia. Methods Twelve rat models of chronic hind limb ischemia established by unilateral femoral artery ligation were randomized into PEO and control groups (n=6) and treated with intravenous infusion of PEO and saline through the internal jugular vein every other day for 2 weeks. Contrast-enhanced ultrasonography was performed after the treatments to evaluate the blood flow in the skeletal muscles at different time points and blood flow reserve in the ischemic hind limbs on day 28. Results Starting from 7 days after femoral artery ligation, blood flow in the ischemic hind limb skeletal muscles was significantly higher in PEO group than in