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In vitro toxicity assessment of extracts derived from sol–gel coatings on polycarbonate intended to be used in food contact applications.pdf
Food and Chemical Toxicology 93 (2016) 51e57
Contents lists available at ScienceDirect
Food and Chemical Toxicology
journal homepage: /locate/foodchemtox
In vitro toxicity assessment of extracts derived from solegel coatings on polycarbonate intended to be used in food contact applications
Isabelle Severin d, *, Krystelle Lionti b, Laurence Dahbi d, Catherine Loriot c, Berangere Toury a, Marie-Christine Chagnon d
a Laboratoire des Multimateriaux et Interfaces UMR 5615, University of Lyon, Villeurbanne, 69100, France b IBM Almaden Research Center, San Jose, CA 95120, USA c Laboratoire National de Metrologie et dessais, Trappes 78197, France d Derttech ? Packtox ? LNC UMRU866, Nutox Team, Agrosupdijon, Universite Bourgogne-Franche-Comte, Dijon, 21000, France
article info
Article history: Received 4 March 2016 Received in revised form 28 April 2016 Accepted 29 April 2016 Available online 30 April 2016
Keywords: Ames test Micronucleus assay Endocrine disruption HepG2 cell line Solegel coating Food contact coating Polycarbonate Migration Extracts
abstract
Polycarbonate is a widely used polymer in food contact applications all around the world. However, due to the potential release of Bisphenol A (BPA) during repeated washing cycles, its use becomes compromised as BPA is known for being an endocrine disruptor for rodents. In order to tackle this issue, solegel coatings based on organoalkoxysiloxane were developed on PC, to act as a physical barrier. To this end, two solegel systems based on tetraethylorthosilicate (TEOS), methyltriethoxysilane (MTES) and 3-glycidyloxypropyltriethoxysilane (GPTES), three common solegel precursors, were prepared. The coatings derived from the latter two systems were then studied with regards to their potential toxicity in vitro. Migration tests were performed in food simulants, and the maximal migration was obtained in ethanol 10% (v/v) for one system and in isooctane for the other one. In vitro genotoxicity was assessed with the Ames
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