有哪些信誉好的足球投注网站Human Tear Fluid Reduces Culturability of Contact Lens-Associated Pseudomonas aeruginosa Biofilms but Induces Expression of the Virulence-Associated Type III Secretion System.pdfVIP
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Human Tear Fluid Reduces Culturability of Contact Lens-Associated Pseudomonas aeruginosa Biofilms but Induces Expression of the Virulence-Associated Type III Secretion System.pdf
Original Research
ALI DJALILIAN, MD, EDITOR
Human Tear Fluid Reduces Culturability of Contact Lens-Associated Pseudomonas aeruginosa Bio?lms but Induces Expression of the Virulence-Associated Type III
Secretion System
YVONNE T. WU, OD, PHD, 1 CONNIE TAM, PHD, 1* LUCIA S. ZHU, BA, 1 DAVID J. EVANS, PHD, 1,2 AND SUZANNE M.J. FLEISZIG, OD, PHD1,3
ABSTRACT Purpose: The type III secretion system (T3SS) is a signi?cant virulence determinant for Pseudomonas aeruginosa. Using a rodent model, we found that contact lens (CL)-related corneal infections were associated with lens surface bio?lms. Here, we studied the impact of human tear ?uid on CL-associated bio?lm growth and T3SS expression. Methods: P. aeruginosa bio?lms were formed on contact lenses for up to 7 days with or without human tear ?uid, then exposed to tear ?uid for 5 or 24 h. Bio?lms were imaged using confocal microscopy. Bacterial culturability was quanti?ed by viable counts, and T3SS gene expression measured by RT-qPCR. Controls included trypticase soy broth, PBS and planktonic bacteria. Results: With or without
Accepted for publication September 2016. From the 1School of Optometry, University of California, Berkeley, CA, 2College of Pharmacy, Touro University California, Vallejo, CA and 3Graduate Groups in Vision Science, Microbiology, and Infectious Diseases Immunity, University of California, Berkeley, CA, USA.
This research was supported by the National Institutes of Health, EY011221 (SMJF), an American Optometric Foundation Vistakon Research Grant (YW/SMJF), and by the Australian National Health and Medical Research Council CJ Martin Fellowship (YW).
The authors have no commercial or proprietary interest in any concept or product described in this article.
Single-copy reprint requests to Suzanne M.J. Fleiszig, OD, PhD (address below).
Corresponding author: Dr. Suzanne M.J. Fleiszig, OD, PhD, School of Optometry, University of California, Berkeley, CA 94720. Tel: 1 (510) 643-0990. Fax: 1 (510) 643-5109. E
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