Effect of IFN-γ, IL-12 and IL-10 cytokine production and mRNA expression in tuberculosis patients with diabetes mellitus and their household contacts.pdfVIP

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Effect of IFN-γ, IL-12 and IL-10 cytokine production and mRNA expression in tuberculosis patients with diabetes mellitus and their household contacts.pdf

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Effect of IFN-γ, IL-12 and IL-10 cytokine production and mRNA expression in tuberculosis patients with diabetes mellitus and their household contacts.pdf

Cytokine 81 (2016) 127–136 Contents lists available at ScienceDirect Cytokine journal homepage: /cytokine Effect of IFN-c, IL-12 and IL-10 cytokine production and mRNA expression in tuberculosis patients with diabetes mellitus and their household contacts Ponnana Meenakshi a, Sivangala Ramya a, Joshi Lavanya a, Valluri Vijayalakshmi a,b, Gaddam Sumanlatha a,c,? a Department of Immunology, Bhagwan Mahavir Medical Research Center, Hyderabad, India b Department of Immunology and Molecular Biology, LEPRA Society, Hyderabad, India c Department of Genetics, Osmania University, Hyderabad, India article info Article history: Received 22 November 2015 Received in revised form 3 March 2016 Accepted 9 March 2016 Keywords: Tuberculosis with diabetes mellitus Household contacts Cytokine production Fold change Antigen85A abstract Objective: The study was carried out to understand the in?uence of IFN-c, IL-12 and IL-10 cytokine production and expression in tuberculosis patients with diabetes mellitus (TBDM) and their household contacts (HHC). Methodology: The study involved a total of 300 subjects, 50 in each category of TBDM, TBDM HHC, pulmonary tuberculosis patients (PTB), PTB HHC, DM and healthy controls (HC). TBDM, PTB and their HHC, 25 each were followed at different intervals to determine their immune responses in Ag85A stimulated culture supernatants by Enzyme Linked Immunosorbent Assay (ELISA). mRNA expression by TRIZOL method in 5 cases of each category and follow-up studies were performed. Results: IFN-c and IL-12 cytokine production markedly decreased and that of IL-10 increased after Ag85A M.tb stimulation, however anti TB treatment reconstituted the response in TBDM and PTB patients. The household contacts revealed cytokine gene expression similar to that of patients and two of them developed the disease during follow-up. Conclusion: Cytokine responses of the patients retained after treatment highlighting the antigen importance, hence further studies with recombinant