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Digitalization of a Wide Field Contact Specular Microscope.pdf
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ScienceDirect
IRBM 37 (2016) 103–108
Digitalization of a Wide Field Contact Specular Microscope
G. Hor a,?, Y. Gavet b, A. Bernard a, C. Urrea c, P. Gain a,c, G. Thuret a,c,d
a Corneal Graft Biology, Engineering and Imaging Laboratory, EA2521, Federative Institute of Research in Sciences and Health Engineering, Faculty of Medicine, Jean Monnet University, St-Etienne, France
b Ecole Nationale Supérieure des Mines de Saint-Etienne, France c Ophthalmology Department, University Hospital, Saint-étienne, France
d Institut Universitaire de France, Paris, France Received 14 January 2016; received in revised form 29 February 2016; accepted 1 March 2016
Available online 14 March 2016
Abstract
Objectives: Non-contact specular microscopes (SM) have a very small ?eld of observation (usually 0.1 mm2). This constitutes a major limitation when accurate and extended assessment of the corneal endothelium is needed, for instance during clinical trials or dif?cult diagnosis. Previous contact SM had the advantage of a wide ?eld thanks to the use of an applanating cone but none of the old commercial devices passed the stage of digitalization. This paper aims at upgrading a wide ?eld contact SM with a digital camera and dedicated software in order to combine wide ?eld with the advantages of non-contact SM.
Materials and methods: The digital device was composed of an acquisition unit consisting in a contact Konan Specular SP5500 equipped with a digital camera Canon EOS 6D and a Linux-based computer. Image processing uses segmentation algorithms (Gestalt theory and watershed with markers).
Results: The device acquired wide ?eld (0.5 mm2) highly contrasted images. The software provided cell boundaries and statistics: endothelial cell density, coef?cient of variation of cell area and percentage of hexagonality. Contact SM images signi?cantly increased the sampling of endothelial cells and consequently the reliability of endothelial quality analysis.
Conclusion: The di
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