长链非编码RNA的作用机制及其方法_夏天概述.pdf

HEREDITAS (Beijing) 2013 3 , 35(3): 269 280 ISSN 0253-9772 DOI: 10.3724/SP.J.1005.2013.00269 RNA ,, , , 315211 RNA(Long non-coding RNA, lncRNA) , lncRNA RNA RNA mRNA lncRNA lncRNA , Northern - RNA 3, RNA catRAPID RNA (Chromatin isolation by RNA purification, ChIRP) (Combined knockdown and localization analysis of non-coding RNAs, c-KLAN) RNA; ; ; Acting mechanisms and research methods of long noncoding RNAs XIA Tian, XIAO Bing-Xiu, GUO Jun-Ming Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China Abstract: Long non-coding RNAs (lncRNAs) play biological roles through a variety of mechanisms, including genetic imprinting, chromatin remodeling, cell cycle control, splicing regulation, mRNA decay, and translational regulation. LncRNAs are involved in the regulation of gene expression through the above mechanisms in different levels. Establishment and application of research technologies are important in understanding of lncRNAs functions. Microarray, RNA sequencing, Northern blot, real time quantitative reverse transcription-polymerase chain reaction, fluorescence in situ hybridization, RNA interference, and RNA-binding protein immunoprecipitation are major tools of exploring biological functions of lncRNAs. Here, we highlighted three advanced methods, i.e., fast predictions of RNA and protein interactions and domains (catRAPID), chromatin isolation by RNA purification (ChIRP), and combined knockdown and localization analysis of non-coding RNAs (c-KLAN). Keywords: long non-coding RNAs (lncRNAs); acting mechanisms; gene expression regulation; research methods : 2012?08?08; : : 2012?09?20 (, ( 2012A610207), 2011B82014), ( XKL11D2127) ( :, , Tel: 0574 E-mail: summer_wo@ : ,,, E-mail: junmingguo@ : 2012-12-21 13:33:08 URL: /kcms/detail/11.1913.R1333.001.html PY2012004) ( 270 HEREDITAS (Beijing) 2013 35 RNA(Long non-coding RNA, lncRNA)