血必净对内毒素休克大鼠心肌TNFα变化的影响.docVIP

血必净对内毒素休克大鼠心肌TNF-α变化的影响 陈洁,姜虹,朱也森 (上海交通大学医学院附属第九人民医院,200011) 目的 本实验通过建立大鼠内毒素休克模型,旨在探讨血必净注射液对休克大鼠心肌细胞肿瘤坏死因子(TNF-α)变化的影响，为临床脓毒症休克的复苏治疗提供相关依据。 方法 本研究选用Wistar成年雄性大鼠65只，随机分为四组，即正常组(5只)、模型组(20只)、治疗组Ⅰ(20只)和治疗组Ⅱ(20只)；采用腹腔注射大肠杆菌脂多糖(LPS)方法制备大鼠内毒素休克模型，除正常组外，其余三组均腹腔注射LPS15mg/kg,注射完毕后即刻内毒素组大鼠予以皮下注射生理盐水10ml/kg抗休克治疗，而治疗组1和治疗组2则分别皮下注射血必净注射液5ml/kg+生理盐水5ml/kg和10ml/kg,正常组大鼠经腹腔注入等量生理盐水。除正常组外其余四组大鼠按不同时相（腹腔注射后30min、2h、6h）分为3个亚组，在不同时相点留取股静脉血标本以及心室肌标本，通过ELISA法测定TNF-α值。 结果 内毒素组大鼠静脉血标本于30min时间点出现TNF-α值高峰，2h和6h时有所下降，其中30min的TNF-α值与正常组及对照组相比存在明显差异(P0.01)。 结论　1.内毒素休克可导致心肌细胞炎症信号通路的激活，导致TNF-α的分泌高峰出现。2.炎症信号通路的激活可以加重心肌本身的自身免疫性炎症损伤。3.血必净注射液可以抑制TNF-α的分泌和表达，减轻心脏本身的炎症反应。 关键词　　血必净注射液,内毒素休克,肿瘤坏死因子(TNF-α) EFFECT OF XUEBIJING ON TNF-α OF CARDIAC MUSCLE IN ENDOTOXIN SHOCK RATS ABSTRACT Objective By producing the rat models with endotoxin shock,to explore the effects of XUEBIJING on the changes of TNF-αof cardiac myocytes in endotoxin shock rats and offer a relevant evident on resuscitation treatment of endotoxin shock. Methods The study choose sixty-five male Wistar rats randomized into four groups:Normal group(n=5);Experimental group(n=15);Therapeutic groupⅠ(n=15);Therapeutic groupⅡ(n=15).Endotonxin shock model was performed by intraperitoneal injection with LPS.Experimental group and Therapeutic group Ⅰand Ⅱ were injected with LPS(15mg/kg) through peritoneal cavity .Experimental group were injected saline(10mg/kg) subcutaneously after LPS injection and Therapeutic group Ⅰand Ⅱ were injected Xuebijing(5ml/kg or 10ml/kg) subcutaneously after LPS injection.Normal group were given the same volume saline by intraperitoneal injection. Except for Normal group,the other three groups were divided into three subgroups according to different timepoint( LPS intraperitoneal injection 30min，2h,6h).Venous blood and cardiac muscle were also obtained.The ELISA was applied to investigated the change of TNF-α. Results The value of TNF-αreached the peak at 30min timepoint in Experimental group