超帅气深蓝课件.pptxVIP

Significance Mechanism Experiment Procedure Results SIGNIFICANCE OsFRDL 4 Al3+ Al3+ Al3+ Al3+ Al3+ ? ? ? Isocitrate OA Citrate Malate CH2OHCOOH CHOCOOH CH3COCOOH Malate Oxalate GA L-ASC TA TCA Cycle KGA Amino Acid Citr- ate MECHANISM ? ? ? The Function of OA Channel Protein Fe Citrate Divide into groups Morphology Biochemistry Molecular Biology Ex OA En OA HPLC OsFRDL1 OsFRDL2 OsFRDL4 Root Elongation Control pH=6.0 IM pH=6.0 DM pH=6.0 NaCl pH=6.0 CuSO4 pH=6.0 Acid pH=4.5 AlCl3 pH=4.5 48H Ex OA 48 Hours Later 40Hz 90s HClO4+K2CO3 Amderlite IR-120B Dowex 1*8 pH=2.1 HClO4, 1.0 mL/min 210nm According to our OBSERVATION, the lengths of all test groups are SIGNIFICANT DIFFERENCE comparing to CON except “pH = 4.5”. And THE RANK from the longest to the shortest is: CON ≈ pH4.5 Al Na ≈ IM DM ≈ Cu When pH=4.5, roots elongation becomes significance longer. The regression shows the link between the secretion and the content of citrate acid. Salt stress makes it a little different that the content of citrate acid isn’t increasing as the secretion. Acid stress cause the remotion of the lines. The expression under Cu and salt stress is significant higher. The expression under Cu, salt, Al, acid stress is significant higher. The expression under Cu, salt, Al, stress is significant higher. CONCLUSION Acid, Al, Cu, DM, IM, Salt Stress can all effect the secretion of Citrate, and the expression of 3 kinds of OA channel protein, Cu can effect the transport of Citrate. The expression of OsFRDL1 have no links with the secretion of Citrate. The expression of OsFRDL2 have a connection of H+ transporter. The expression of OsFRDL2 have a connection of Na+ transporter. Thank U For Your Listening!!! Thank U For Your Listening!!! All Rights for Tony Huang. If you want to use it or part(s) of it, please Email to threecifanggen@live.cn Great appreciation for teachers’ helps in the lab, including H. F. Qian, Z. W. Fu, Y