Modifications of collagen-based biomaterials with immobilized growth factors or peptides》.pdfVIP

Methods 84 (2015) 44–52 Contents lists available at ScienceDirect Methods journal homepage: www.else /locate/ymeth Modifications of collagen-based biomaterials with immobilized growth factors or peptides Yun Xiao a,b, Lewis A. Reis b, Yimu Zhao a, Milica Radisic a,b,c,⇑ a Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada b Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada c Toronto General Research Institute, University Health Network and IBBME, University of Toronto, Toronto, ON, Canada a r t i c l e i n f o a b s t r a c t Article history: In order to provide an instructive microenvironment to facilitate cellular behaviors and tissue regenera- Received 11 January 2015 tion, biomaterials can be modified by immobilizing growth factors or peptides. We describe here our pro- Received in revised form 20 April 2015 cedure for modification of collagen-based biomaterials, both porous scaffolds and hydrogel systems, with Accepted 21 April 2015 growth factors or peptides by covalent immobilization. Characterizations of the modified biomaterials Available online 4 May 2015 (immobilization efficiency, release profile, morphology, mechanical strength, and rheology) and in vitro testing with cells are also discussed. Keywords: