Molecular cloning and characterization of interferon regulatory factor 9 (IRF9) in Japanese flounder, Paralichthys olivaceus》.pdfVIP

Molecular cloning and characterization of interferon regulatory factor 9 (IRF9) in Japanese flounder, Paralichthys olivaceus》.pdf

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Molecular cloning and characterization of interferon regulatory factor 9 (IRF9) in Japanese flounder, Paralichthys olivaceus》.pdf

Fish Shellfish Immunology 39 (2014) 138e144 Contents lists available at ScienceDirect Fish Shellfish Immunology journal homepage: www.el /locate/fsi Short communication Molecular cloning and characterization of interferon regulatory factor 9 (IRF9) in Japanese flounder, Paralichthys olivaceus Guo-Bin Hu a,b, *, Ming-Yu Zhao b, Jing-Yun Lin b, Qiu-Ming Liu b, Shi-Cui Zhang a, b a Institute of Evolution Marine Biodiversity, Ocean University of China, Qingdao 266003, China b College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China a r t i c l e i n f o a b s t r a c t Article history: Interferon regulatory factor 9 (IRF9) in mammals is known to be involved in antiviral response. In this Received 14 March 2014 study, we studied the structure, mRNA tissue distribution and regulation of IRF9 from Japanese flounder, Received in revised form Paralichthys olivaceus. The cDNA sequence of IRF9 is 3305 bp long, containing an open reading frame 10 April 2014 (ORF) of 1308 bp that encodes a peptide of 435 amino acids. The predicted protein sequence shares 33.7 Accepted 2 May 2014 e72.0% identity to other fish IRF9s. Japanese flounder IRF9 possesses a DNA-binding domain (DBD), an Available online 14 May 2014 IRF association domain (IAD), two nuclear localization signals (NLSs) and a proline-rich domain (PRD). The IRF9 transcripts were detectable in all examined tissues of healthy Japanese flounders, with higher Keyw

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