Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family》.pdfVIP

Toxicology 229 (2007) 245–252 Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family Cong Luo a , Yongliang Zhub , Tiejun Jiang a , Xiaoyong Lub , Wo Zhang a , Qifeng Jing a , Jia Li a , Linrong Pang a , Kangjie Chen a , Fuming Qiu a , Xiuyan Yu a , Jiahui Yang a , Jian Huang a,∗ a Cancer Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China b Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China Received 25 August 2006; received in revised form 17 October 2006; accepted 30 October 2006 Available online 28 November 2006 Abstract Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. © 2006 Elsevier Ireland Ltd. All rights reserved. Keywords: Matrine; Apoptosis; Bcl-2 family 1. Introduction 2004). Some studies indicated that Matrine could induce