保加利亚乳杆菌和嗜热链球菌生物膜形成研究王坤.pdf

184 2011, Vol. 32, No. 19 食品科学 ※生物工程 保加利亚乳杆菌和嗜热链球菌生物膜形成研究 王 坤，闫颖娟，姜 梅，陈晓红，李 伟，董明盛* (南京农业大学食品科技学院，江苏南京 2 10095) 摘 要：采用置片法和菌落计数法对嗜热链球菌(Strep tococcus thermop hilus)和保加利亚乳杆菌(Lactobacillus bulgaricus) 在不同载体表面( 椰果粒、不锈钢网布、塑料片、陶瓷片和玻璃片) 上形成生物膜的能力进行研究。结果表明：椰 果粒和不锈钢网布是适宜乳酸菌单菌生物膜形成的载体，培养7d 椰果粒和不锈钢网布上的单菌生物膜初始菌密度可 7 2 6 2 7 2 达10 CFU/cm ，并且在后续培养中能稳定在3.2 ×10 CFU/cm 左右；混菌生物膜菌密度连续7d 稳定在1 ×10 CFU/cm 左右。利用扫描电镜对S. thermop hilus 和L . bulg aricus 形成的混菌生物膜进行观察，结果表明在椰果粒和不锈钢网 布表面上S. thermop hilus 和L . bulg aricus 形成典型的混菌生物膜结构。 关键词：乳酸菌；生物膜；载体；稳定性 Biofilm-forming Capabilities of Lactobacillus bulgaricus and Strep tococcus thermop hilus WAN G Kun ，YAN Yin g-j u an ，JIANG Mei ，CHEN X iao-hon g ，LI Wei ，DONG Min g-shen g* (College of Food Science and Technology, Nanj ing Agricultural University, Nanj ing 2 10095, China) Abstract ：The biofilm-forming capabilities of Strep tococcus thermop hilus and Lactobacillus bulgaricus on different carriers (coconut, stainless steel mesh, plastic sheet, glass sheet or ceramic parts) was studied by using placed slice and plate culture count methods. The results showed that stainless steel mesh and coconut were suitable carriers for biofilm formation. During 7 days, the CFU (colony-forming units) of the single-species biofilm on coconut and stainless steel mesh initially reached 1 ×107 2 6 2 7 2 CFU/cm and then was kept at 3.2 ×10 CFU/cm . The mixed-species biofilm wa