基因quot;慢quot;的克隆及其在小鼠组织中的表达.pdfVIP

Acta Physiologica Sinica , August 25, 2005, 57 (4): 433-438 433 Research Paper Expression of man, a formin homology domain-containing gene in the mouse limb 1,* 2 3 CUI Yan-Zhen , PENG Yi-Feng , TIAN Qi 1Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Science University, 20000 NW Walker Rd., Beaverton, OR 97006, USA; 2Institute of Reproductive Medicine, Yijishan Hospital, Wannan 3 Medical College, Wuhu 241001, China; Department of Pathology, L113, School of Medicine, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA Abstract: Formin defines a family of structurally related proteins that have two formin homology domains (FH1 and FH2). Various mutations in the formin locus result in limb deformity, suggesting that these genes play indispensable roles in the limb development. Here we report the isolation of a novel cDNA, man, from the mouse limb, which contains two conserved FH1 and FH2 domains. Its expression is described and possible functional significance is discussed. Key words: man; formin; FH1; FH2; mouse limb; cytokinesis; expression cloning 1,* 2 3 1 2 97006 3 97239 241001 Formin formin (formin homology domains)FH1 FH2 FH1 FH2 man formin formin FH1 FH2 Q956 The first two alleles of the mouse limb deformity (ld) mains (FH1 and FH2), although some members in this mutation, which were isolated in the 1960s, are among