Surface Etching and DNA Damage Induced by Low-Energy Ion Irradiation in Yeast.pdfVIP

Surface Etching and DNA Damage Induced by Low-Energy Ion Irradiation in Yeast.pdf

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Surface Etching and DNA Damage Induced by Low-Energy Ion Irradiation in Yeast.pdf

PlasmaScienceandTechnology,Vo1.13,No.3,Jun.2011 SurfaceEtching and DNA Dam ageInduced by Low-Energy Ion Irradiation inYeast LIUXuelan(刘雪兰),XUAn(许安)。,DAIYin(戴银),YUANHang(袁航)。 YUZengliang(余增亮)。 KeyLaboratoryofZoonosesofAnhuiProvincejAnhuiAgriculturalUniversity, Hefei230036,China InstituteofPlasmaPhysics,ChineseAcademyofSciences,Hefei230031)China AbstractBio—effectsofsurvivalandetchingdamageoncellsurfaceandDNA strandbreaks wereinvestigatedintheyeastsaccharomycescerevisiaeafterexposurebynitrogenionwithanen— ergYbelow 40keV.Theresultshowedthat16%oftrehaloseprovideddefiniteprotectionforcells againstvacuum stresscomparedwithglycero1.Incontrasttovacuum control,significantmorpho— logicaldamageandDNA strandbreakswereobserved.inyeastcellsbombardedwithlow—energy nitrogen,byscanningelectronmicroscopy (SEM)andterminaldeoxynucleotidyltransferase— mediatedduTPnickendlabeling(TuNEL)immunofluorescenceassays.Moreover,PI(propidium iodide1fluorescentstainingindicatedthatcellintegritycouldbedestroyedbyionirradiation.Cell damageeventually affected cellviabilityandfreeradicalswereinvolved in celldamageasshown byDMsO (dimethyIsulfoxide)rescueexperiment.Ourprimaryexperimentsdemonstratedthat yeastcellscan beused asan optionalexperimenta1modeltostudythebiologicaleffectsoflow energyionsandbeappliedtofurtherinvestigatethemechanism(s)underlyingthebio—effectsof eukaryoticcells. Keywords:low—energynitrogenions,yeast,radiationmodel,damageeffects PACS:41.75.A.C 1 Introduction at30。CinYPDmediumconsistingof1%yeastextract

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