牛奶中三价铬和六价铬的同时提取和检测方法.pdfVIP

250 2010, Vol. 31, No. 10 食品科学 ※分析检测 牛奶中三价铬和六价铬的同时提取和检测方法 李 静，王 雨，陈华宝，梁立娜* (戴安中国有限公司应用研究中心，北京 100085) 摘 要：建立牛奶中三价铬和六价铬的同时提取和测定方法。前处理过程中一次完成六价铬的提取、有机铬中三 价铬的释放、三价铬柱前衍生以及牛奶蛋白沉降。铬的提取效率高，牛奶基体去除效果好，操作简单方便。使 用IonPac CS5A 色谱柱可以在8min 内完成牛奶中两种铬的价态分析，三价铬和六价铬分别以PDCA 和DPC 作为络 合剂，分别在紫外和可见波长下采用UV -V is 检测器进行检测，灵敏度高，基体干扰小。为牛奶的质量检测提供 了准确实用的方法。 关键词：三价铬；六价铬；离子色谱；U V -V i s 检测；牛奶质量检测 Simultaneous Extraction and Determination of Chromium (Ⅲ) and Chromium (Ⅵ) in Milk LI Jin g ，WANG Yu ，CHEN Hu a-bao ，LIANG Li-n a* (Application Research Center, Dionex China, Beij ing 100085, China) Abstract ：A method was developed for the simultaneous extraction and determination of chromium (Ⅲ) and chromium (Ⅵ) in m ilk . One pretreatment p rocess w as requ ired for chromiu m (Ⅵ) extraction, the release of chromiu m (Ⅲ) from org anic chromium, chromium (Ⅲ) pre-column derivatization and milk protein sedimentation, which exhibited simplicity, convenience and high extraction efficiency of chromium. The separation of chromium (Ⅲ) and chromium (Ⅵ) was achieved on an IonPac CS5A column within 8 min . Chromium (Ⅲ) and chromium (Ⅵ) were chleated with PDCA and DPC and were detected with a UV-Vis detector at UV and visible wavelengths, respectively . This work provides an accurate and practical method for milk quality evaluation . Key w ords：chromium (Ⅲ)；chromium (Ⅵ)；ion chromatograp hy ；UV-Vis detection ；milk quality testing 中图分类号：O657.32 文献标识码：A 文章编号：1002-6630(20 10)10-0250-04 日前，某乳业公司多批次牛奶中被检出一种名为 原子吸收和发射光谱等方法只能检测铬的总量；直 “皮革水解蛋白粉”的物质，该物质的生产原料是制 接分光光度法检测时两种铬精确度难以保证。有文献报 革厂的边角废料，由于制革过程中需要大量的三价铬作