Preliminary Study on Geographical Variation of Cytochrome b gene and ITS2-rDNA among Populations of Rhynchophorus ferrugineus.pdfVIP

Preliminary Study on Geographical Variation of Cytochrome b gene and ITS2-rDNA among Populations of Rhynchophorus ferrugineus.pdf

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Preliminary Study on Geographical Variation of Cytochrome b gene and ITS2-rDNA among Populations of Rhynchophorus ferrugineus.pdf

JournalofAgriculturalScienceandTechnologyB1(2011)189-197 Earliertitle:JournalofAgriculturalScienceandTechnology,ISSN 1939-1250 煞 … 蟛 PreliminaryStudyonGeographicaIVariationof CytochromebgeneandITS2-·rDNA amongPopulations ofRhynchophorusferrugineus R.A.A.M.E1.Mergawy一,A.M.A1Ajlan,N.Abdalla4,V.Vassiliou,C.Capdevielle.Dulac,D.C. Kontodimas.J.F.Silvain andM .I.Nasr ,IRD,UR072,LaboratoireEvolution,GdnomesetSpdciation,UPR9034,CNRS,Gif-sur—Yvette91198,France 2DepartmentofMolecularBiology,GeneticEngineeringandBiotechnoloyg ResearchInstitute(GEBRI),MinoufyiaUniversity, El-SadatCity,Minoufyia,Eygpt 3.DepartmentofAridLandAgriculture,CollegeofAgriculture&FoodSciences,KingFaisalUniversity,POBox55009,Hofuf 31982,KingdomofSaudiArabia 4DepartmentofGenetics,FaculytofAgriculture,CairoUniversiyt,Eygpt 5.AgriculturalResearchInstitute,P.0.Box22016,1516Nicosia,Cyprus BenakiPhytopathologicalInstitute,P0.Box51214,Kfifissia,Greece 7。UniversitdPnris—SudllOrsayCedex91405.France Received:July21,2010 /Published:June20,2011 Abstract:TheRedPalmWeevil(RPW),Rhynchophorusferrugineus(Olivier),(Coleoptera,Curculionidae,Rhynchophorinae),isan invasivespeciesthatisoriginatedfrom SoutheastAsia.IthasinvadedM iddleEast,severalcountriesoftheMediterraneanBasin, CaribbeanfIslandofCuracaoNetherlandAntilles),LebanonandUnitedStatesofAmerica(LagunaBeach,OrangeCounty,Califomia) during theIastthree decadeswhere itattacksPalm trees.Thisstudy investigatedthegeographicalvariation among geographic populationsofRPW collectedrfom twelveinvadedcountriesusingcytochromeb(Cytb)andITS2partialsequences.Thecomparison amongtheCytbsequencesresultedinthreedifierenthaplotypesdesignatedasHBltoHB3.Thethreehaplotypesweresubdividedinto twophylogeneticgroupsaccordingtotheirgeographicpositions:(1)the“Multi—Continent”groupcontainingHBIhaplotypedetectedin eightpopulationsbelonging tothreedifferentC

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