PPH治疗环状内痔82例体会.docVIP

PPH治疗环状内痔82例体会 　　作者：邓华瑜,罗红霞,范维珂　　作者单位：重庆医科大学病理生理学教研室,重庆 　　提　要:目的　研究HSP90的表达与乳腺癌细胞侵袭转移能力的相关性。方法　采用RT-PCR和Western blot检测 　　HSP90在MDA-MB-435s和MDA-MB-231细胞中mRNA水平和蛋白质水平的表达差异。Transwell小室人工重组基底膜侵袭迁移实验检测经HSP90抑制剂Geldanamycin(GA)处理前后两株细胞侵袭迁移能力的改变。结果　MDA-MB-435s细胞中HSP90Α的mRNA表达水平明显高于MDA-MB-231细胞(P0. 01), MDA-MB-435s和MDA-MB-231细胞中HSP90Β的mRNA表达水平没有明显差异(P0. 05), MDA-MB-435s细胞中HSP90的蛋白质表达水平明显高于MDA-MB-231细胞(P0. 01)。两株细胞用药前侵袭迁移能力无明显差异(P0. 05),HSP90的抑制剂GA对MDA-MB-435s和MDA-MB-231细胞的侵袭迁移能力均呈现明显抑制作用(P0. 01,P0. 05),对MDA-MB-435s细胞的抑制作用更明显(P0o01)。结论　HSP90的表达影响乳腺癌细胞侵袭转移能力。 　　关键词:乳腺癌细胞株,HSP90,侵袭转移 　　Abstract:Objective　To investigate the correlation between theHSP90 expression and invasion andmetastasis potential ofhuman breastcancer cell line.Methods　ThemRNA expression levelofHSP90wasdetected by RT-PCR. The protein expression level ofHSP90 was measured byWestern blotting. The effects ofHSP90 inhibitorGeldanamycin(GA)on the invasion and motility abilities of human breast cancer lineMDAMB-231 andMDA-MB-435sin vitrowere explored by transwell chamber.Results　ThemRNA expression level ofHSP90ΑinMDA-MB-435s cellwas higher than hat ofMDA-MB-231 cell(P0. 01). The mRNA ex-pression levelofHSP90Βhad no difference(P0. 05)between the two cell lines. The protein expression levelofHSP90 inMDA-MB-435s cellwas higher than thatofMDA-MB-231 cell(P0. 01). The invasive andme-tastatic potential in human breastcancer cellMDA-MB-435s andMDA-MB-231 had no difference(P0. 05).After treated with GA,the invasive and metastatic potential in human breast cancer cellMDA-MB-435s andMDA-MB-231 was suppressed significantly(P0. 01,P0. 05),but the effects ofHSP90 inhibitorGA on in-vasive andmetastatic potentialofMDA-MB-435s cellwasmore significant than thatofMDA-MB-231 cell(P0. 01).Conclusion　The abilities of invasion andmetastasis ofbreastcancer cells are related to the expressionofHSP90. 　　Key words:breast cancer line;HSP90;metastasis 　　乳腺癌是妇女最常见的恶性肿瘤之一,转移扩散是其预后不良的重要原因,一些乳腺癌患者局部肿瘤控制多年后,仍可发生血行转移[1]。热休克