HPLC的使用.docVIP

April 19Version 1.02 (minor additions and correcti We will use High Performance Liquid Chromatography (HPLC) to separate acetylsalicylic acid, caffeine, and acetaminophen in commercially available pain relievers.我们将使用高效液相色谱法（HPLC）分离市售的止痛药乙酰水杨酸，咖啡因和对乙酰氨基酚。 You must choose a product that has at least two of the above ingredients in it.你必须选择一个产品，至少有两个在它上面的成分。 The package should give you the manufacturers composition.该软件包应该给你制造商组成。 At the conclusion of the analysis you can compare your results with those stated on the package.在分析的结论，你可以比较你的结果，与包装上指明的。 Dont assume that the package is correct.不要以The purpose of the written report is to establish the credibility of yo The structures of the compounds we are separating are given below.下面给出我们分离的化合物的结构。 One more compound has been added, Salicylic Acid.一个化合物已增加，水杨酸。 This substance will be our Internal Standard, and will be added in constant amount to all our standard and unknown runs.这种物质将我们的内部标准，将在不断的金额加入到我们所有的标准和未知的运行。 The peak at 3 minutes might be acetaminophen or caffeine and that at 8 minutes could be one of the two acids, but dont count on it. 3分钟，高峰期可能是对乙酰氨基酚或咖啡因，8分钟可两个氨基酸之一，但不要指望它。 You have to devise a way to identify your peaks once you have achieved baseline separation.你必须制定一种方法，一旦你已经达到基线分离，以确定您的峰。 This is where standards come in. We will be preparing standard solutions of acetaminophen, acetylsalicylic acid, caffeine and salicylic acid.这是地方标准进来，我们将准备标准溶液，对乙酰氨基酚，乙酰水杨酸，咖啡因和水杨酸。 These will be combined to create a series of calibration solutions that will allow us to determine which peak is which, and what the relationship is between the peak area and the concentration of the standard.这些都将结合创建一系列的校准解决方案，将使我们能够确定峰，峰面积和浓度的标准之间的关系是什么。 The Instrument仪器 Part of the standard lab report is identification and description of the actual equipment you have used, so make sure you get the manufacturer and model number of all equipment, as well as the source (state the manufacturer, not the stuff in the lab) and characteristics (r