HPLC测定黄芩正气胶囊中橙皮苷_黄芩苷_和厚朴酚_厚朴酚的含量.pdfVIP

200 6 1 中成 药 Janu ary 2006 28 1 Ch inese T rad itiona l P aten t M ed icine V o l 28 N o 1 HPLC 谢友良, 曾惠芳, 林 吉, 吴秋云, 曾 宝, 黄耀海, 苏子仁* ( 广州中医药大学 广东 广州 510405) : ; ; ; ; ; : : () : Phenom enex luna C18 ( 2) , 0 2% ; : 294 nm; : 35 C: HPLC , , 99 25% , RSD 1 0 34% ; 9 8 70% , RSD 3 05% ; 99 32% , RSD 2 44% ; 100 07% , RSD 2 67% : , : R 927 2 : A : 2006) 0100340 3 Determination ofhesperidin, baicalin, honokiolandmagnolol in HuangqinZhengqiCapsulebyHPLC * X IE Y ouliang, ZENG H u ifang, L IN Jie, W U Q iuyun, ZENG Bao, HUANG Y aoh ai, SU Z iren (Guangzhou University of TraditionalCh ineseM edicine, Guangzhou510 05, Ch ina) KEY ORDS: H u angqin Zhengqi Cap su le; hesp erid in; b aicalin; honok io;l m agno lo l; H PLC ABSTRACT: AIM: T o estab lish a m e thod o f determ in ing effect ive com ponents in H u angqin Zhengqi C apsule ( R ad ix Scutellariae, P ericarp ium C itri R eticu latae, Cortex M agnoliae O ffic inalis, etc ) METHODS: T he conten ts of hesp erid in, ba icalin, honokio l and m agno lol w ere determ ined by H PLC The chrom a tographic cond ition s w ere: Phenom enex luna C18 ( 2) column, u sing m eth ano l0 2% phospho ric acid solution as a m ob ile phase, detecting w ave leng th at 294 nm and the co lum n tem perature being 35 C RESULTS: T he four effective com ponents cou ld be detected by this m ethod, and their contents could be determ ined A good linearity of hesperidin, b aicalin, honoki ol and m agnolo l w as in the range of 0 1440 864 g, 0 249 61 497 6 g, 0 1000 600 g, 0 0980 588 g, re sp ective ly; the average recoveries w ere 99 25% , 98 70% , 99 32% , 100