果用型银杏叶黄酮内酯类成分资源化学评价.pdfVIP

果用型银杏叶黄酮与内酯类成分资源化学评价 1 2 2 姜玮 ，姚鑫 ，唐于平 1 扬州市药品检验所，江苏扬州 225000； 2 南京中医药大学江苏省方剂研究重点实验室，江苏南京 210046 [摘要] 目的: 探索不同树龄、产地、性别果用银杏叶中总黄酮与总内酯的含量变 化。方法：采用HPLC-PDA和HPLC-ELSD分别测定果用银杏叶中总黄酮与总内 酯的含量。结果：果用银杏叶中总黄酮与总内酯的含量随年龄和产地变化差异较 大，不同树龄银杏叶中总黄酮与总内酯的含量树龄大的较低，树龄小的较高；雄 树银杏叶总黄酮与总内酯含量高于雌树银杏叶总黄酮与总内酯含量；地理因素对 果用银杏叶总黄酮与总内酯含量影响较大。结论：不同树龄、产地、性别果用银 杏叶中总黄酮与总内酯的含量变化规律研究为我国银杏资源的综合利用与开发 提供了科学依据。 [关键词] 果用银杏叶；总黄酮；总内酯；HPLC-ELSD ；HPLC-PDA Comparative Characterization of Total Flavonol Glycosides and Terpene Lactones in Different Ages, Cultivation Sources and Gender of Ginkgo biloba Leaves 1 2 2 JIANG WEI ,YAO XIN , TANG YU-PING 1 2 Yangzhou FDA, Yangzhou Jiangsu 225000； Jiangsu Key Laboratory for High Technology of TCM formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China OBJECTIVE: Exploring the content change of total flavonoids and total lactonesthe in different age, origin and gender of G. Biloba leaves. METHODS: Using the HPLC - pdas and HPLC - ELSD to calculate the content of total flavonoids and total lactones in G. Biloba leaves. RESULTS: The content of total flavonoids and total lactones changed with the age and origin, the content of total flavonoids and total lactones in old G. Biloba leaves was lower than the young; the content of the told flavonoids and total lactone in male G. Biloba leaves was higher than the female; Geographical factors had great influence on the content of total flavonoids and total lactones. CONCLUSION: The study of the content change rules of the total flavonoids and total lactonesthe of G. biloba provided the scientific basis for the comprehensive utilization and development of G. biloba in China. KEY WORDS: G. biloba leaves; terpene lactone; flavonol glycoside; HPLC-ELSD; HPLC-PDA 1.引言 银杏是古老的树种，具有活化石之称[1,2] 。银杏叶提取物由于确切的疗效而 [3-4] 被广泛用于中草药与保健品 。越来越多的证据表明银杏叶提取物对心脑血管 [5-7] 疾病和老年性痴呆有一定的疗效 。银杏叶富含黄酮苷、内酯、双黄酮和原花 色素，黄酮苷和内酯为其主要的活性物质基础