应用荧光原位杂交技术检测上尿路尿路上皮癌可显著提高诊断敏感性.pdfVIP

( ) JOURNAL OF PEK ING UN IVERSITY (HEALTH SCIENCES) Vol. 4 No. 4 Aug. 010 381 论著 应用荧光原位杂交技术检测上尿路的尿路上皮癌 可显著提高诊断敏感性 穆大为, 周利群 , 丁 义, 何志嵩, 王 莹, 何 群, 杨新宇 ( , , 100034) [ ] 目的: ( fluorescence in situ hybrid ization, FISH ) , FISH 方法: 007 1 008 6 , 0, 3717 p16, FISH , 63 69 , FISH , FISH , M cNem ar结果: 37 17 p16 1. 7% . 4% 1. 0% 1. 3% FISH 84. 1% ( 53/63) 41. 3% ( 6/63)FISH PT PT PT 71. 4% ( 0/ 8) 94. 3% ( 33/ a 1 - 4 35) , PT PT PT 35. 7% ( 10/ 8)45. 7% ( 16/35); a 1 - 4 , FISH 80. 0% ( 0/ 5) 44. 0% ( 11/ 5), 86. 8% ( 33/38) 39. 5% ( 15/38)FISH 91. 3% ( 63/69) 94. % ( 65/69) 结论: FISH , FISH , , [ ] ; ; , ; [ ] R737. 15 [] A [ ] 1671167X( 010) do:i 10. 3969/ .j issn. 1671167X. 010. 04. 005 Clinical utility of fluorescence in situ hybridization improves the sensitivity in the diagnosis ofupper urinary tract urothelialcarcinoma MU Daw ei, ZHOU Liqun , DING Y i, HE Zh i song, WANG Y ing, H E Qun, YANG Xinyu ( Departm ent ofU rology, PekingUn iversity F irstH ospita;l Institute ofU rology, PekingUn iversity, Beijing 100034, Ch ina) ABSTRACT Objective: To assess the clin ical utility of a fluorescence in situ hybridization ( FISH ) assay as a noninvasivemethod for d iagnosing and monitoring urothelial carcinoma (UC) in the upper urinary tract (UUT ). Methods: U rine specmi ens from 63 consecutive patientsw ith UUTUC and 69 controls w ith ben ign disease w ere analyzed by means of cytology and FISH. For FISH analysis, labeled probes specific for chromosomes 3, 7, and 17 and for the p16 ( 9p 1) genew ere used to as