Three-dimensional reconstruction of light microscopy image sections present and future.pdfVIP
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Three-dimensional reconstruction of light microscopy image sections present and future.pdf
Front.Med.2015,9(1):3 _45
!!: : 箜鬯鬯
Three-dimensionalreconstructionoflightmicroscopyimage
sections:presentandfuture
YuzhenWang,P.uiXu,GaoxingLuo,JunWu(函)
InstituteofBurnResearch,SouthwestHospital,StateKeyLaboratoryofTrauma,Burns,andCombinedlnjury,ChongqingKeyLaboratory
,.DiseasesProteomics,theThirdMilitary MedicalUniversity,Chongqing40003 China
◎HigherEducationPressandSpringer-VerlagBerlinHeidelberg2014
Abstract Three-dimensional(3D)imagereconstructiontechnologiescanrevealpreviouslyhiddenmicrostruc.
turesinhuman tissue.However,thelack ofideal,non.destructivecross—sectionalimagingtechniquesisstilla
problem.Despitesomedrawbacks.histologicalsectioningremainsoneofthemostpowerfulmethodsforaccurate
high-resolution representation oftissuestructures.Computertechnologiescan produce3D representatiOns0f
interestinghumantissueandorgansthathavebeenserial-sectioned,dyedorstained,imaged,andsegmentedfor
3Dvisualization.3Dreconstructionalsohasgreatpotentialinthefieldsoftissueengineeringand3DPrinting.This
articleoutlinesthemostcommonmethodsofr3D tissuesectionreconsrtucti0n.W edescribethemostimportant
academicconceptsinthisfield andprovidecriticalexplanationsandcomparisons alsonotekeystepsinthe
reconsrtuctionprocedures,andhighlightrecentprogressinthedevelopmentofnew reconstructionmethods.
Keywords microtomy;3D imaging;computer-assistedimageprocessing;3D printing;tissuescaffold
lntroduction andtissuefrom amicrocosmicperspective,suchasmicro—
computedtomography(microCT),microscopicmagnetic
M icroscopicinvestigationofhumanorgansandtissuewas resonancemiaging(microMRI),andconfocallaserscan—
oncerestrictedtothetwo—dimensional(2D、leve1.How. ningmicroscopy(CLSM),arerevolutionizinghistological
ever,todescribeallentiresampleusing2D imagedata, evaluationinthelifescience
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