配伍减毒提高银杏叶制剂安全性研究的论文.pdfVIP

配伍减毒提高银杏叶制剂安全性研究 潘苏华，刘平平，李寰舟 (南京中医药大学中药学一级学科，江苏南京，210046南京210029) 摘要：目的：探讨配伍法提高银杏叶制剂安全性。方法：采用高效液相法和小鼠口服毒性法比较了刺梨与银杏叶 提取物配伍前后银杏酸含量变化及小鼠死亡率相关性；用配位化学法观察了银杏酸与金属离子的配位程度；用 Cocktail探针药物法比较了单复方银杏叶制剂对酒精性肝损伤大鼠血浆CYP2E1活性的影响。结果：刺梨与银杏 叶提取物配伍，可降低制剂中银杏酸的含量，且与小鼠口服毒性降低趋势相一致；银杏酸可与金属离子Fe3+／Cu2+ 发生不同程度的配位；复方银杏叶制剂可抑制酒精刺激性肝损伤大鼠血浆CYP2EI活性的升高，从而减轻肝细胞氧 化应激损伤。结论：刺梨与银杏叶配伍可提高银杏叶制剂的安全性，其机制与银杏酸相关。 [Abstract]OBJECTIVETo the of biloba method． investigatesafetyginkgo bycompatibility in biloba and METHODDeterminethecontentsof acidsthe of extracts ginkgolic preparationGinkgo Rose withthe HPLC．Theacute wasobserved roxburghiicompared prescriptionby toxicity preceding after doseof the of bilobaextractsandRosa inmice． single ig preparationGinkgo Roxburghii Determinethecoordinationbetween acidsandmetalionscoordinmion degree ginkgolic by chemistry． Evaluatetheeffectsof bilobaonCYP2E1 inexperimental rats compoundginkgo activity hypedipidemia Cocktail The ofRose and bilobaextracts by rox-burghiiGinkgo probedrugs．RESULTcompatibility reducedthecontentsof acidsinthe concentrationshave consistent ginkgolic preparations．The great with acidswith withthereductionof inmice oraladministration．Thecoordinationof toxicity ginkgolic metalionsFe3+／Cu2+in bilobacouldinhibitactivitiesofCYP2E1 varyingdegrees．Compoundginkgo