分子生物学方法龋病.docVIP

上海第二医科大学 教 案 课程名称 口腔生物学 第 6 次课 2008 年 3 月 26 日 教师姓名 王丽珍 职称 讲师 教研室 基础 (口腔生物学) 教学时数 3 授课题目 分子生物学研究的主要方法 及龋病分子生物学 教学对象 口腔医学院04级 授课地点 口腔医学院102教室 教学方式 理论课 本次的重点、难点及对策： Key Points: Gene Clone Structure-Function Relationship of the GTFs Difficulties and Countermeasures: Gene Clone Countermeasure: First, explain the definition of “Clone” and “Gene Clone”, then explain the processes of gene clone technique. Virulence Factors in Cariogenic Streptococcus Mutans Countermeasure: Explain the definition of “virulence factors” at first, and then explain the virulence factors in cariogenic streptococci, especially the adherence. Functions of Glucosyltransferases in Streptococcus Mutans Countermeasure: First, clear the relationship between dental caries and streptococci mutans. Second, explain how the glucosyltransferases split sucrose and form glucans. Last, definitude the functions of Glucosyltransferases. Structure-Function Relationship of the GTFs Countermeasure: First, divide the GTFs protein into three domains, and introduce the function of each domain. Then, correspond each protein domain to its gene domain, and explain the function of each gene domain. 本次课应用的教材教具参考书： 教材：刘正主编。口腔生物学。第三版。国家级规划教材，卫生部规划教材 教具：多媒体、黑板、话筒 主要参考书： 陈竺、王亚新主编。分子生物学与疾病。百家出版社 分子生物学。Robert F. Weaver。科学出版社 姜泊、张亚历、周殿元主编。分子生物学常用实验方法。人民军医出版社 金冬雁等译。分子克隆实验指南。科学出版社 主要教学内容及时间分配： Part I Fundaments Of Molecular Biology (5 minutes) What is molecular biology? What genes are made of? How do genes work? How genes are replicated? How genes direct the production of polypeptides? How genes accumulate mutations? How important molecular biology is in oral research? Part II Major Techniques Of Molecular Biology (45 minutes) Gene cloning Definition What is clone? What is gene cloning? Procedure How to construct a gene library? Genomic library cDNA library How to isolate the desired gene? Application of polynucleotides/oligonucleotides probes Application of antibodies How to analyse the