ATP-TCA抗肿瘤药物敏感性检测技术与复发或难治NHL临床治疗的相关性研究.pdfVIP

ATP-TCA抗肿瘤药物敏感性 检测技术与复发或难治NHL 临床治疗的相关性研究 杨 磊宋诸臣徐小红蒋 斌彭春雷魏金芝 南通市肿瘤医院内科，南通226361 摘要：采用三磷酸腺苷生物荧光法(ATP-TCA)对复发或难治非霍奇金淋巴瘤组织标本进行体外药敏试验，并分 析其临床应用价值。方法：应用ATP-TCA技术对复发或耐药淋巴瘤患者术后新鲜组织或胸腹水标本共34例进行体 外药敏试验。结果：淋巴瘤标本的药物敏感性具有个体差异性，不同化疗药物的体外抑瘤活性不同。ATP-TCA组总 有效率(RR)为82.4％，完全缓解率(CR)为52.9％；DICE化疗组RR为63.6％，CR为40.9％；GDP化疗组RR为 62.3％，CR为26.4％。ATP-TCA组的有效率分别与两对照组相比P均＜0.05。结论：ATP-TCA体外药敏检测结果与临 床治疗反应有很好的相关性，是开展肿瘤个体化化疗的一种重要的体外药物筛选方法。 关键词：三磷酸腺苷生物荧光法；非霍奇金淋巴瘤；体外药敏试验 CORRELATIONBETWEENATP BIOLUMINESCENCETUMOR CHEMOSENSITIVITYASSAYAND CLINICALRESPONSEINRECURRENT ANDREFRACTORYNHL YANGLeiSONGZhuchengXUXiaohongJIANGBingPENGChunlei WEIJinzhi DepartmentofInternalMedicine,NantongTumarHospital,Nantong226361,China Abstract:TodeterminethecorrelationbetweenresultsofATPbioluminescencetumorchemosensitivityassay(ATP-TCA)ofhu manrecurrentandrefractoryNHLspecimensinvitroandclinicalchemo-therapeuticresponsesofpatients.Methods:Thirty-four freshlytakenrecurrentandrefractoryNHLspecimensweretestedinvitroforcancerchemosensitivitybyATP-TCA.Results:Drug sensitivityofNHLspecimenshaveheterogeneity.Differentdrugshasdifferenttumorgrowthinhibitionratioinvitro.Cilnicaloutcome ofthepatientsreceivingchemotherapyaccordingtoinvitroassaywasasfollowing:responserate(RR,CR+PR)was82.4％,complete responserate(CR)was52.9％.InDICEcaseresponseratewas63.6％,completeresponseratewas40.9％.InGDPcaseresponserate was62.3％,completeresponseratewas26.4％.Incontrolcaseswhichweresignificantlypoorerthanthatintestgroup(P＜0.05).Con clusion:TheresultsofATP-TCAassayarecorrelatedwellwithclinicaltreatmentresponses.Theassaymaybeanimportantand usefulmethodforindividual-based