2%2c5-己二酮致大鼠卵巢颗粒细胞凋亡及其基因表达.pdfVIP

(P0.05)。 3.各染毒组Bcl-2 mRNA表达均低于对照组，差别有统计学意义(P0.05)。各 染毒组Bcl-xL mRNA表达与对照组比较，差别无统计学意义(P0.05)。60 mmol/L 组Xiap mRNA表达低于对照组，差别有统计学意义(P0.05)。40 mmol/L组Bax mRNA 表达明显高于对照组，差别有统计学意义(P0.05)。各染毒组FasL mRNA表达均 高于对照组，差别有统计学意义(P0.05)。 4.40和60 mmol/L组卵巢颗粒细胞的NF- κB p65蛋白表达显著下降，与对照 组比较, 差别有统计学意义(P0.05)。60 mmol/L组NF- κB p65蛋白转位活化水 平高于对照组，差别有统计学意义(P0.05)。 【结论】 1.在本实验条件下2,5-己二酮呈现对大鼠卵巢颗粒细胞明显的毒性作用，表 现为卵巢颗粒细胞活力的降低、卵巢颗粒细胞凋亡的增加。 2.Bax、FasL基因表达的上调和Bcl-2、Xiap基因表达的下调可能是2,5-己二 酮致使大鼠卵巢颗粒细胞发生凋亡的重要机制之一。 3.2,5-己二酮导致的卵巢颗粒细胞凋亡可能与NF- κB p65蛋白表达量的下 降以及活性的增加，并进而影响凋亡相关基因的表达有关。 【关键词】 正己烷 2,5-己二酮 卵巢颗粒细胞 凋亡 基因 2 Apoptosis of Rat Ovarian Granulosa Cells by 2,5-hexanedione in vitro and its Relevant Gene Expression Abstract Objective 2,5-hexanedione(2,5-HD) is a major metabolite of n-hexane in the body.This study examined the apoptosis effect caused by 2,5-HD and its regulatory mechanisms on the ovarian granulosa cell,in order to provide a reference for the further research on the prevention and control of n-hexane’s effects and the mechanisms of female reproductive toxicity. Methods Ovarian granulosa cells were took out from immature rats and incubated in vitro. After cellular adherence occurred, the cells were randomly divided into four groups and added with cell culture media containing 2,5-HD,which final concentration was 0,20,40,and 60 mmol/L, respectively. The culturing continued and was not treated until the exposure ended. 1. Effect of 2,5-HD on ovarian granulosa cells’ viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl-tetrazolium bromide(MTT) assay after 0-hour, 12-hour, 24-hour, and 36-hour exposure. 2. The cellular morphological changes were observed through a common optical microscope