甘薯花青素的提取及其抑菌效果探究.pdfVIP

中国农业科学 2005,38(11):2321-2326 Scientia Agricultura Sinica 甘薯花青素的提取及其抑菌效果分析 王关林，岳 静，李洪艳，方宏筠 （辽宁师范大学生命科学学院，大连116029） 摘要：建立了甘薯块根花青素（sweetpotato anthocyanin,SPAC）的大孔树脂吸附制备技术，分析了其抑菌 作用和机理。结果表明，在0.8%柠檬酸、物料比1∶200，60℃温水浴提取2 h的条件下可获得最大的提取率3.81 -1 mg·g 。 粗提物经 AB-8 大孔树脂过柱纯化后可获得色价为 225.1E 的纯品，色价提高 6 倍。研究发现 SPAC 对 3 种常见致病菌均有抑菌作用，并与其浓度呈正相关。通过电镜观察、SDS分析及生长曲线比较表明，SPAC 抑 菌机理是通过SPAC与细胞中的蛋白或酶结合，使其变性失活，抑制对数生长期的细胞分裂，而后使细胞质固缩、 解体，导致细胞死亡。SPAC具有色素和抑菌双重功能，是理想的功能性天然色素。 关键词：甘薯；花青素；提取；抑菌 Extraction of Anthocyanin from Sweetpotato by Macroporous Resin and Its Bacteriostatic Mechanism WANG Guan-lin, YUE Jing, LI Hong-yan, FANG Hong-jun (College of Life Science,Liaoning Normal University, Dalian 116029) Abstract: The extraction technology by using adsorption of macroporous resin and the bacteriostatic mechanism of anthocyanin from sweetpotato were studied in an experiment. The results showed that the maximum extraction rate of anthocyanin from sweetpotato 3.81 mg·g-1 was gained on the optimal extractive conditions of 0.8% citric acid, the material to solvent ratio of 1: 200, 60oC homothermal bainmarie, 2 hours extraction time. The high purity extracts with 225.1E colour value ( E1%1cm532nm ) was obtained through purification by using AB-8 macroporous resin’s adsorption, which was six times as the crude extracts. The inhibitory effects of anthocyanin from sweetpotato on growth of E.coli, S.aureus and P.aeruginos were discovered, and showed a positive correlation with the concentration of anthocyanin from sweetpotato. Observation under transmission electron microscope, SDSanalysis and comparing growth curves of normal E.coli affected by anthocyanin from sw