大鼠脑基底动脉最适初始张力的确定.docVIP

  大鼠脑基底动脉最适初始张力的确定# 肖雪，平娜娜，李森，曹蕾，曹永孝** （西安交通大学医学院药理学系） 5 10 15 20 25 30 35 40 摘要：目的：确定 SD 大鼠脑基底动脉收缩的最适初始张力。方法：将大鼠脑基底动脉制成 1~2 mm 长的环段，用两根钢丝悬挂于 Myograph 恒温浴槽中。将 IC1/IC100 值设置为 0.6~1.0， 记录其相对应的初始张力。在不同的初始张力下，记录 60 mM KCl 诱导的收缩力，研究诱 导的最大收缩力与初始张力的关系。结果：随着 IC1/IC100 值从 0.6 至 1.0，脑基底动脉初始 张力逐渐从 0.47±0.01 mN/mm 增加到 2.68±0.03 mN/mm，对 IC1/IC100 和初始张力进行相 关性分析，相关系数为 0.95，表明二者正相关。KCl 诱导的收缩力先随 IC1/IC100 值升高而 升高，当 IC1/IC100 值为 0.9、初始张力为 1.63 mN/mm 时，KCl 诱导的收缩力达到最大，其 值为 6.51±0.05 mN/mm，明显高于 IC1/IC100 在 0.6 和 0.7 时的收缩力（P0.05）。此后， 随着 IC1/IC100 值的增加，KCl 诱导的收缩力不再增加，反而呈下降趋势。结论：大鼠脑基 底动脉环的最适初始张力为 1.63±0.01 mN/mm。 关键词：药理学；脑基底动脉; 初始张力；肌张力描记系统；大鼠 中图分类号：R965.2 An optimal initial tension of rat basilar artery in wire myography XIAO Xue, PING Nana, LI Sen, CAO Lei, CAO Yongxiao (Department of Pharmacology, Xi’an Jiaotong University College of Medicine, Xian 710061) Abstract: Objective: The present study was to determine the optimal initial tension in rat basilar artery. Methods: Rat basilar arteries were cut into approximately 1~2 mm, threaded on two thin wires (40 μm in diameter), and mounted in myograph baths. Normalization procedure was carried out. IC1/IC100 was set from 0.60 to 1.00, and the initial tensions were recorded. KCl 60 mM was added into the baths to induce a contractile tension. Results: The initial tension of basilar artery increased from 0.47±0.01 mN/mm to 2.68±0.03 mN/mm as the value of IC1/IC100 increased from 0.6 to 1.0. The correlation coefficient between IC1/IC100 and initial tension was 0.95. At first, the contractile tension was elevated with the value of IC1/IC100 and initial tension increasing. When the IC1/IC100 and initial tension reached to 0.9 and 1.63 mN/mm, the contractile tension reached its maximum value, 6.51±0.05 mN/mm. Then the contractile tension started to decrease as IC1/IC100 or initial tension increased. The contractile tensions in IC1/IC100 value of 0.9 was significantly higher than in the IC1/IC100 value of 0.6 and 0.7 (P0.05). Conclusion:.The mo