色谱的五个塔板数之间的关系.pdfVIP

中国科技论文在线 色谱的五个塔板数之间的关系 1 2 2* 张大力 ，柯家骏 ，卢立柱 （1. 北京联合大学生物化学工程学院，北京 100023； 2. 中国科学院过程工程研究所，北京 100080 ） 摘要：在塔板理论的基础上，应用色谱滑移机理研究探讨了五种塔板数计算公式之间的相互 关系。论文提出色谱研究中应该区分色谱柱塔板数与色谱峰塔板数这两个概念，以避免理论 研究中的某些难题。用色谱峰塔板数解释实验色谱峰的对称性，化解了塔板理论的一个难题， 即巨大的理论塔板数对应于显著拖尾的实际色谱峰的矛盾。这五种塔板数是指四种色谱柱塔 板数和一种色谱峰塔板数。四种色谱柱塔板数分别是理论塔板数、真实塔板数、塔板模型塔 板数和滑移机理塔板数。 关键词： 色谱机理，塔板理论，滑移机理，色谱柱塔板数，色谱峰塔板数 中图分类号：O647，O657 Relationship of five plate numbers of chromatography 1 2 2 ZHANG Dali , KE Jiajun , LU Lizhu (1. Biochemical Engineering College of Beijing Union University, Beijing 100023; 2. Institute of Process Engineering, Chinese Academy of Science, Beijing 100080) Abstract: Slip mechanism and plate theory were applied to the investigation of the relationship of computing formulas of five plate numbers. It was proposed in this paper that chromatography researchers should distinguish the difference between column plate number and peak plate number in order to avoid some difficult problems in theoretical research. The peak plate number was used to the explanation for the peak form, which resolved one difficult problem in plate theory, the contradiction of a seriously tailing peak with a very big column plate number. The five plate numbers meant four kinds of column plate numbers and one kind of peak plate number; the four column plate numbers meant theoretical plate number, real plate number, plate-model plate number and slip-mechanism plate number. Key words: chromatographic mechanism, plate t