纳米银颗粒的合成、表征及抑菌性能的研究.docVIP

 纳米银颗粒的合成、表征及抑菌性能的研究 徐罕琦，钱和，庞月红** （江南大学食品学院，江苏 无锡 214122） 5 10 摘要：采用紫外光还原法制备纳米银颗粒。采用紫外-可见光谱(UV-vis)、荧光光谱(FL)、傅 立叶变换红外光谱(FT-IR)、X 射线衍射(XRD)、透射电镜(TEM)、能量衍射光谱(EDS)和圆 二色谱(CD)表征纳米银颗粒的形貌特征、光学特性、晶体结构、元素组成等。并在此基础上 以大肠杆菌和金黄色葡萄球菌为受试菌株，用抑菌圈法、最小抑菌浓度(MIC)和最小杀菌浓 度(MBC)测试本实验合成的纳米银颗粒的抑菌、抗菌性能。结果表明：硝酸银与 L-半胱氨 酸的摩尔比为 1.5:1，光照时间为 2.5 h 时，制备的纳米银颗粒粒径小且分布比较均匀（5~10 nm），形态为球形或类球形，对两种受试菌株有很好的抑菌、杀菌效果，MIC 均为 0.0438 mg/mL，MBC 则为 0.350 mg/mL。 关键词：纳米银颗粒；紫外光还原法；抑菌 中图分类号：TS201.6 15 Synthesis and characterizations of silver nanoparticles and its antibacterial property XU Hanqi, QIAN He, PANG Yuehong (School of Food Science and Technology,Jiangnan University, JiangSu WuXi 214122) 20 25 30 35 40 Abstract: Silver nanoparticles were synthesized by UV irradiation.The synthesized silver nanoparticles were characterized by ultraviolet–visible spectroscopy (UV-vib), fluorescence (FL), fourier-transform infrared (FTIR), spectrum and X-ray diffraction(XRD), transmission electron microscopy (TEM), energy dispersive spectrometer (EDS) and circular dichroism (CD). Taking Escherichia coli and Staphylococcus aureus as target strains, inhibition and antibacterial effect of silver nanoparticles was measured by inhibition zone, MIC and MBC. The results demonstrated that silver nanoparticles are spherical with a narrow size distribution, whereas the diameter of the individual particles are between 5 and 10 nm. The silver nanoparticles have the better magnetism under 2.5 h UV light when the mole ratio of silver nitrate and L-cysteine is 1.5∶1. The inhibition and antibacterial effect of silver nanoparticles was excellent. The MIC and MBC to both strains are 0.0438 mg/mL and 0.350 mg/mL. Key words: silver nanoparticles; UV photoreduetion; antibacterial property 0 引言 纳米粒子是指直径在 1~100 nm 之间的粒子，也称为超微粒子。由于具有小尺寸效应、 表面效应、量子尺寸效应和宏观量子隧道效应，因而显示出不同于常规材料的热、光、电、 磁、催化和敏感等特性。作为纳米贵金属的代表，纳米银以其在超导、光电[1-2]、抗菌[3]、 催化[4]等领域潜在的应用价值而受到人们的广泛关注。作为无机抗菌材料，纳米银由于其原 子排列表现为介于固体和分子之间的“介态”，其比表面积极大，表面效应、小尺寸效应和 宏观隧道效应明显，具有其它载银无机抗菌材料以及抗生素无法比拟的抑菌、杀