缺铁与光对不同玉米自交系DMA昼夜分泌规律的影响.docVIP

 缺铁与光对不同玉米自交系 DMA 昼夜分泌 规律的影响# 董炳雪，李岩，张春庆，吴承来，孙爱清** 5 10 15 20 25 30 35 40 （山东农业大学农学院，作物生物学国家重点实验室，山东省作物生物学重点实验室） 摘要：为了研究不同玉米自交系 DMA 昼夜分泌规律是否一致，选用 12 个玉米自交系进行 缺铁处理，结果表明玉米 DMA 分泌不存在昼夜节律，在缺铁条件下 DMA 分泌量比正常条 件下的显著提高，昼夜分泌规律不同品种间存在显著差异（tt11,0.05）。根据分泌高峰的 数量将品种分为 2 类，一类具有一个分泌高峰（7:00-10:00），一类会在傍晚（19:00-22:00） 出现第二个分泌高峰，可能原因是是缺铁胁迫反馈作用于光暗因子，这些因子又作用于负责 PS 分泌的蛋白，促使 DMA 又一次大量分泌。采用 6 个材料进行遮光的处理，收集时遮光 不会影响 DMA 昼夜分泌规律（tt7,0.05），说明在短暂遮光情况下玉米根系 DMA 分泌不 受光照的影响；收集前遮光 24h 也没有影响 DMA 昼夜分泌规律（tt7,0.05）），但 DMA 分泌量明显升高，说明 DMA 的合成可能主要是在前一天黑暗中进行的，遮光处理使 DMA 合成量增加，从而使 DMA 分泌增加。 关键词：玉米自交系；缺铁；DMA；昼夜变化 中图分类号：S513 Probing the effects of Fe-deficiency and light on diurnal variation of phytosiderophore release in maize inbred DONG Bingxue, LI Yan, ZHANG Chunqing, WU Chenglai, SUN Aiqing (State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University) Abstract: The effects of the iron (Fe) nutrition status and time of the day on the release of 2’-deoxymugineic acid (DMA) in roots were studies over a 24-hour cycle for twelve maize inbred lines. The result showed that the specie didn’t show a distinct diurnal rhythm in DMA secretion and had low but constant release. Compared with the Fe-sufficient plants, the amount of DMA secretion of 12 maize inbred improved significantly in iron-deficiency plants. Different varieties showed up different secretion rules（tt11, 0.05）。These inbred lines can be divided into two classes according to the numbers of PS secretion peak. One has only one secretion peak at 7:00-9:00 and the other has two secretion peaks consisted of prime peak occurred at 7:00-10:00 and secondary peak occurred at19:00-22:00. The reason for appearing the second peak was probably that Fe-deficient stress caused feedback effect on light factors which then acted on the proteins responsible for PS secretion and resulting in DMA secreting a lot once again. Shading treatment during collection did not affect the secretion rule in