妊娠大白猪和梅山猪母胎界面TLR4的表达模式比较研究.docVIP

 Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan 5 10 15 20 25 30 35 40 45 pigs# Liu Huazhen1, Zhang Gaoying1, Peng Kemei1, Liu Yinxue1, Yu Mei2** (1. College of Animal Science and Veterinary Medicine, Huazhong (Central China) Agricultural University, WuHan 430070; 2. College of Animal Science and Veterinary Medicine, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong (Central China) Agricultural University, WuHan 430070) Abstract: Toll-like receptor 4 (TLR4) has been suggested to play a very important role in modulation of immunological tolerance in the human female reproductive tract. To better understanding of functions of TLR4 in establishment and maintenance of pregnancy in pigs, the study was performed to compare the expression patterns of TLR4 at maternal-fetal interface of pregnant Yorkshire and Meishan pigs. The uteri from Yorkshire and Meishan gilts on days 26 and 50 of gestation were obtained respectively. Immunohistochemical analysis indicated that TLR4 was detected in uterine epithelium and trophoblast of these two types of pigs on days 26 and 50 of gestation. In Yorkshire gilts, the expression of TLR4 was higher in uterine epithelium than that in trophoblast on day 26 of gestation, while it was lower in uterine epithelium than that in trophoblast on day 50 of gestation. In Meishan pigs, the expression of TLR4 in trophoblast was higher than that in uterine epithelium on either day 26 or day 50 of gestation and TLR4 was found to be mainly present in the cells located at the bottom of trophoblast on day 50 of gestation. Compared with Meishan pigs, TLR4 was abundant in uterine epithelium of Yorkshire pigs on days 26 and 50 of gestation, yet it was decreased in trophoblast of Yorkshire pigs on day 50 of gestation. The results indicated that TLR4 at the maternal-fetal interface is expressed in differential patterns between York