大粒车前子中阿拉伯木聚糖结构分析.docVIP

 大粒车前子中阿拉伯木聚糖结构分析# 殷军艺1，聂少平1，林慧霞1，Cui Steve W.1,2，谢明勇1** （1. 南昌大学食品科学与技术国家重点实验室，南昌 330047； 5 10 2. 加拿大农业与农业食品部，Guelph 食品研究中心，Guelph N1G5C9） 摘要：PLP-3 是从大粒车前子中提取和纯化的多糖组分，利用单糖组成分析、部分酸水解， 并结合甲基化、FT-IR、GC、GC-MS、1D 和 2D NMR 对其结构进行解析。结果显示 PLP-3 是阿 拉伯木聚糖，含有葡萄糖醛酸。PLP-3 以β-1,4-linked Xylp 顺序连接为主链，通过 O-2（占 17.87%）或 O-3（占 24.24%）与支链相连。主要末端残基有 T-linked Araf（8.13%）、T-linked Xylp（15.58%）和 T-linked GlcAp（13.22%），其它残基还有 1,2,5-linked Araf、1,2-linked Rhap、T-linked Glcp 和 T-linked Galp 等。综合上述分析，推断出 PLP-3 可能的重复结构 单元。 关键词：大粒车前子；阿拉伯木聚糖；结构；甲基化；NMR 中图分类号：O652.4; R284.2 15 Structural analysis of arabinoxylan from the seeds of Plantago asiatica L. Yin Junyi1, Nie Shaopin1, Lin Huixia1, Cui Steve W.1,2, Xie Mingyong1 (1. State Key Laboratory of Food Science and Technology, Nanchang University, 20 25 30 35 40 NanChang 330047; 2. Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, N1G 5C9, Canada) Abstract: PLP-3 (Plantago asiatica L. polysaccharide 3) was isolated and purified from the seeds of Plantago asiatica L.. Its structure characters were elucidated by monosaccharide composition analysis, partial acid hydrolysis and methylation analysis, combined with FT-IR, GC/MS, 1D and 2D NMR spectroscopy. PLP-3 was found to be arabinoxylan, containing glucuronic acid. It was consisted of a β-1,4-linked Xylp backbone with short side chains attached to its O-2 (1,2,4-linked Xylp, 17.87%) or O-3 (1,3,4-linked Xylp, 24.24%) positions. The main terminal residues were T-linked Araf (8.13%), T-linked Xylp (15.58%) and T-linked GlcAp (13.22%). Small amounts of other residues were also found in PLP-3, such as 1,2,5-linked Araf, 1,2-linked Rhap, T-linked Glcp and T-linked Galp, ect. A possible molecular structure was proposed. Keywords: Plantago asiatica L.; arabinoxylans; structure feature; methylation; NMR 0 引言 大粒车前子是车前子中的一种，广泛分布于中国，在江西吉安一带有大量种植。最近的 研究表明，大粒车前子中来源的多糖具有较好的诱导树突状细胞成熟[1,2]和抗氧化活性[3]。 大粒车前子多糖经分离、纯化后得到 PLP-1（18.9%）、PLP-2（52.6%）和 PLP-3（28.5%） 三个组分，其中 PLP-2 糖含量（87.32%）高，而蛋白