超级稻花后强、弱势粒蛋白质表达的差异.docVIP

 超级稻花后强、弱势粒蛋白质表达的差异# 陈婷婷，谈桂露，褚光，刘立军，杨建昌** （扬州大学江苏省作物遗传生理重点实验室，江苏扬州 225009） 5 10 15 20 25 30 35 40 摘要：为进一步揭示水稻强、弱势粒灌浆差异的机理，本试验以两个超级稻品种两优培九（两 系杂交籼稻）和淮稻 9 号（粳稻）为材料，通过双向电泳观察花后强、弱势粒蛋白质表达的 差异并对差异蛋白进行质谱分析。结果表明，弱势粒花后 3～15 d 的灌浆速率和最终粒重均 显著低小于强势粒。花后 3 d、8 d 和 15 d 弱势粒中蛋白质表达的点少于强势粒，花后 25 d 则弱势粒多于强势粒。花后强、弱势粒间差异蛋白质表达量在 2 倍以上的点有类似变化趋势。 选择有显著差异的 27 个蛋白点进行质谱分析，其中 14 个点的功能得到鉴定。这些蛋白质分 别参与籽粒的淀粉合成、蛋白合成、光合代谢、能量代谢、环境适应以及细胞信号转导等。 说明强、弱势粒间多种蛋白质表达的差异是其灌浆和粒重差异的重要原因。 关键词：超级稻；强/弱势粒；蛋白质表达；蛋白质功能；籽粒灌浆 中图分类号：S511 Difference in Protein Expressions between Superior and Inferior Spikelets of Super Rice after Anthesis CHEN Tingting, TAN Guilu, CHU Guang, LIU Lijun, YANG Jianchang (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009) Abstract: Poor grain filling of the later-flowering inferior spikelets (in contrast to the earlier-flowering superior spikelets) is a serious problem in rice production. This problem is more aggravated in new bred super rice cultivars. To better understand the mechanism underlying the poor grain filling of inferior spikelets, This study investigated protein expressions in superior and inferior spikelets after anthesis through two-dimensional gel electrophoresis (SDS) and the MALDI analysis and by using two super rice cultivars, Liangyoupeijiu (indica hybrids) and Huaidao 9 (japonica). Results showed that the grain filling rate at 3-15 d after anthesis (DAA) and the final grain weight of inferior spikelets were much smaller than those of superior spikelets. The spots of protein expressions were fewer in inferior spikelets than in superior spikelets at 3, 8, and 15 DAA, and the results were reversed at 25 DAA. The spots of protein expressions with two-fold difference between superior and inferior spikelets showed a similar tendency. The twenty seven protein spots that showed significant difference in the expressions between superior and inferior spikelets were chosen for the MALDI analysis, of which 14 protein spots we