羧基单壁碳纳米管的眼内安全性研究.docVIP

 羧基单壁碳纳米管的眼内安全性研究# 王亚平1,2，晏露1,2，侯江平1，陈志1,2，林蜜蜜1,2，张茜1，刘勇1,2** （1. 温州医学院眼视光学院，浙江 温州 325027； 5 10 15 20 25 30 35 2. 温州医学院先进材料与纳米生物技术研究院，浙江 温州 325027） 摘要：目的：通过动物实验观察羧基单壁碳纳米管的眼内生物安全性。方法：选择健康成年 雄性 SD 大鼠，随机分为 3 组，每组 5 只，羧基单壁碳纳米管剂量分别为 0.05 、0.1、 0.2 mg/ml，各 5μl 注入实验眼，各组均以右眼为实验眼，左眼注入等量的磷酸盐缓冲液（PBS） 为对照，共观察 1 个月。采用裂隙灯和间接眼底镜等方法进行临床观察，玻璃体腔注入羧基 单壁碳纳米管后观察眼压变化，视网膜电图评价注入前及注入后各时间点视网膜功能状态， 光镜观察视网膜各层结构改变。结果：玻璃体腔注入羧基单壁碳纳米管前后眼压没有明显的 变化，视网膜电图未见明显的改变，光镜观察视网膜各层结构未见明显改变。结论：实验剂 量的羧基单壁碳纳米管注入 SD 大鼠玻璃体腔未见明显毒性。 关键词：羧基单壁碳纳米管；眼内压；视网膜电图 中图分类号：O657.1 Intraocular biocompatibility of SWCNT-COOH WANG Yaping1,2, YAN Lu1,2, HOU Jiangping1, CHEN Zhi1,2, LIN Mimi1,2, ZHANG Qian1, LIU Yong1,2 (1. School of Ophthalmology and Optometry, Wenzhou Medical College, ZheJiang WenZhou 325027; 2. Institute of Advanced Materials for Nano-Bio Applications, Wenzhou Medical College, WenZhou ZheJiang 325027) Abstract: Purpose: Intraocular biocompatibility of the SWCNT-COOH has been investigated by intravitreal injection of SWCNT-COOH into eyes of SD rats. Methods: SWCNT-COOH dispersions with various concentrations including 0.05mg/5μl, 0.1mg/5μl, 0.2mg/5μl were intravitreally injected in the right eye of the SD rat respectively. Simultaneously PBS solutions were injected into the left eye as a control. Intraocular biocompatibility of the injected SWCNT-COOH was studied using different techniques such as intraocular pressure (IOP), electroretinography (ERG）and histologic examination. Slit-lamp biomicroscopy and fundoscopy were carried out at baseline. IOP was measured after injection of SWCNT-COOH in the first week, over 2 weeks and 4 weeks respectively. ERG tracings were recorded over 2 weeks and 4 weeks after the injection. The experimental rats were subsequently killed and the retinas were examined by light microscopy. Results: IOP and ERG studies showed no significant different between the control and the nanotube-injected eyes at various doses. Histologic examination showed no retinal abnormality in the rats wi