芳香化酶抑制剂对胶炎性关节炎大鼠血清中IL17和RANKL_RANK_OPG的影响.docVIP

 芳香化酶抑制剂对胶炎性关节炎大鼠血清 中 IL17 和 RANKL/RANK/OPG 的影响# 5 10 15 20 25 30 35 40  摘要：目的 探讨第三代芳香化酶抑制剂阿那曲唑对胶原诱导的关节炎（CIA）大鼠血清中 白介素 17（IL17）、核因子-κＢ受体活化因子（RANK）及其配（RANKL）和骨保护素（OPG） 的作用，了解其加重骨关节病变作用及其机制。方法 建立 CIA 大鼠模型，分为阿那曲唑 组、卵巢切除组和假手术组, 用排水法大鼠关节炎足体积变化，放射线分析大鼠关节病变情 况，并用 ELISA 法分析 CIA 大鼠血清中 IL17、RANKL 及 RANK 和 OPG 的表达情况。结 果 卵巢切除组比较，阿那曲唑组大鼠关节病变加重(P0.05)，血清中 IL17、RANKL 表达增 加，OPG 的表达显著降低，RANKL/OPG 比值明显增加，差异均具有统计学意义(P0.05)。 结论 在 CIA 大鼠模型中, 阿那曲唑可增加大鼠血清中 IL17、RANKL 的表达，而抑制 OPG 的表达，导致大鼠关节病变加重。 关键词：芳香化酶抑制剂；胶原性关节炎；IL17；RANKL；OPG 中图分类号：R73 The effect of aromatase inhibitors on IL17 and RANKL/RANK/OPG in serum of collagen induced arthritis rats Wang Jingxuan, Song Ying, Zhao Hong, Jin Shi, Zhao Wenhui, Zhang Qingyuan, Tang Dabei (The Third Hospital of Harbin Medical University, Harbin 150080) Abstract: Abstract: Objective To explore the effect of aromatase inhibitors-anastrozole on IL17 and RANKL/RANK/OPG in the serum of collagen induced arthritis (CIA) rats and to illustrate its aggravation on bone. Methods Induction the model of CIA, the rats were divided randomly into 3 groups: anastrozole group (A); ovarectomy group (O) and sham operation group(S), the edema rate of ankle joints were examined; the radiological injuries of ankle were observed by molybdenum target radiography; the production of IL17 and RANKL/RANK/OPG in the serum were determined by enzyme linked immunosorbent assay (ELISA). Results Compared to ovarectomy group, administration of anastrozole significantly increased the severity of arthritis (P0.05), the levels of IL17 and RANKL in anastrozole group were significantly higher, OPG was lower, while the ratio of RANKL/OPG obviously increased (P0.05). Conclusion: Anastrozole potently promoted the progression of CIA model by the increasing of IL17、RANKL and decreasing of OPG. Keywords: aromatase inhibitors; collagen induced arthritis; IL17; RANKL; OPG 0 引言 第三代芳香化酶抑制剂是绝经后乳腺癌患者主要的内分泌治疗药物，其所致骨关节症状 （关节痛、肌肉痛、骨质疏松和骨折）的发生严重地影响了患者的生活质量，很多患者由于 症状严重不得不放弃芳香化酶抑制剂治疗，影响了内分泌治疗的疗效，所以研究芳香化