免疫学技术在科研中的应用课件.pptVIP

抗原或抗体的检测;抗原抗体反应的特点;抗原抗体反应的影响因素;常用的抗原抗体反应;凝集反应;间接凝集反应;;微粒捕获酶免疫分析技术;;沉淀反应;速率散射比浊法／免疫比浊法;;;免疫印迹技术;免疫印迹法的基本步骤;;免疫标记技术;免疫酶测定法;;ELISA;;酶联免疫斑点试验(enzyme—linkedimmunospotassay，ELISPOT);;;免疫荧光技术;ImmunofluorescentstainofimmunoglobulinG(IgG)showinglinearpatterninGoodpasturessyndrome;天疱疮免疫荧光染色;放射免疫测定法;免疫胶体金技术;;免疫细胞的检测;磁珠分离法;;fluorescence-activatedcellsorter，FACS;;;T细胞鉴定及功能测定;抗原肽—MHC分子四聚体技术tetramer;;B细胞的鉴定及功能测定;细胞因子的检测;基因敲除技术和转基因动物;Th细胞在mCTL介导的肿瘤保护中作用的研究

;相关说明;相关步骤;GenerationofOvalbumin-specific

MemoryCD8TCells.

;;;;;CD8+MemoryTCellsNeedAntigen-specificCD4+T-helperCellstoAchieveTumorProtection

;;Absenceoftumorprotectioninmicewithoutantigen-specificT-helpercellsisnotbecauseoflowerlevelsoftumorantigen(SIINFEKL)-specificCD8+Tcells.;;IncontrasttomCTLs,eCTLsdonotneedThelptokilltumor.

;;Studyofthelong-livedmemoryCD8+TcellsgeneratedinC57BL/6Jmice.

;;;;;1.RoweHM,LopesL,IkedaY,etal.

ImmunizationwithalentiviralvectorstimulatesbothCD4andCD8Tcellresponsestoanovalbumintransgene?

MOLECULARTHERAPY13(2):310-319FEB2006

TimesCited:0

?2.ThompsonJA,DissanayakeSK,KsanderBR,etal.

TumorcellstransducedwiththeMHCclassIItransactivatorandCD80activatetumor-specificCD4(+)Tcellswhetherornottheyaresilencedforinvariantchain?

CANCERRESEARCH66(2):1147-1154JAN152006

TimesCited:0

3.SalucciV,MennuniC,CalvarusoF,etal.

CD8(+)T-celltolerancecanbebrokenbyanadenoviralvaccinewhileCD4(+)T-celltoleranceisbrokenbyadditionalco-administrationofaToll-likereceptorligand?

SCANDINAVIANJOURNALOFIMMUNOLOGY63(1):35-41JAN2006

TimesCited:0

4.RadfordKJ,VariF,HartDNJ

Vaccinestrategiestotreatlymphoproliferativedisorders?

PATHOLOGY37(6):534-550DEC2005

TimesCited:1

5.HuDE,KettunenMI,BrindleKM

MonitoringT-lymphocytetraffickingintumorsundergoingimmunerejection?

MAGNETICRESONANCEINMEDICINE54(6):1473-1479DEC20