正交试验法和响应面法优选蒲公英根甾醇提取.pdf

山东农业大学学报(自然科学版),2022,53(6):913-917VOL.53NO.62022

JournalofShandongAgriculturalUniversity(NaturalScienceEdition)doi:10.3969/j.issn.1000-2324.2022.06.016

正交试验法和响应面法优选蒲公英根甾醇提取

陈倩,王建芳,曹让,杨培志*

西北农林科技大学草业与草原学院,陕西杨凌712100

摘要:优化超声波浸提蒲公英根甾醇的工艺条件。试验控制1.00g样品用20mL乙醇在超声波清洗机里进行浸提，

设计正交试验和响应面试验研究甾醇提取率随着乙醇浓度、超声提取时间、超声提取温度三个因素水平变化而变化

的规律。正交优化分析得出最佳提取工艺下，能提出甾醇含量为46.01mg/g，此时乙醇浓度为80%，提取时间为20

min，提取温度为60℃。响应面分析结果为乙醇浓度87.42%、提取时间10.83min、提取温度59.97℃，这时每克蒲

公英根提取甾醇的含量最高为67.26mg。该结果表明响应面法工艺条件下得到的蒲公英根甾醇提取率高于正交试验

所得。

关键词:蒲公英;甾醇;化合物分离与提取

中图法分类号:S326文献标识码:A文章编号:1000-2324(2022)06-0913-05

SterolExtractingfromTaraxacummongolicumRootbythe

OptimalOrthogonalTestandResponseSurfaceMethodology

CHENQian,WANGJian-fang,CAORang,YANGPei-zhi*

CollegeofGrasslandAgriculture/NorthwestAFUniversity,Yangling712100,China

Abstract:OptimizationofprocessconditionsforultrasonicextractionofTaraxacummongolicumrootsterols.Experimentally

controlled1.00gsampleswereextractedwith20mLofethanolinanultrasoniccleaner.Orthogonalandresponsesurfacetests

weredesignedtoinvestigatethepatternofsterolextractionratewiththechangesinthelevelsofthreefactors:ethanol

concentration,ultrasonicextractiontime,andultrasonicextractiontemperature.Theorthogonaloptimizationanalysis

concludedthatunderthebestextractionprocess,thesterolcontentwas46.01mg/g,andtheethanolconcentrationwas80%,

theextractiontimewas20min,andtheextractiontemperaturewas60℃.Theresultsoftheresponsesurfaceanalysiswere

87.42%ethanolconcentration,10.83minextractiontimeand59.97℃extractiontemperature,andthehigheststerolcontent

of67.26mgperg