基于HPLC-ELSD的巴戟天含量测定方法研究.docxVIP

基于HPLC-ELSD的巴戟天含量测定方法研究

【摘要】目的：通过HPLC-ELSD的巴戟天含量测定方法研究，以耐斯糖和蔗糖进行含量测定，初步确定了对药材巴戟天的质量控制的标准，使得得到一种相对较为快速、简便、有效的测定方法。方法：应用HPLC-ELSD，对巴戟天中耐斯糖和蔗糖进行含量测定。结果：耐斯糖在19.9742μg/mL~59.9226μg/mL浓度范围内线性关系良好，线性方程式为：y=41.873x-576.2，R2=0.9954，精密度和重复性RSD值均小于2%，样品溶液12小时候内稳定性良好；蔗糖在18.0788μg/mL~54.2361μg/mL浓度范围内线性关系良好，线性方程式为：y=51.166x-606.28，R2=0.9965，精密度和重复性RSD值均小于2%；样品溶液12小时候内基本稳定，耐斯糖加样回收率在97.15%~100.15%之间；蔗糖回收率在94.00%~98.85%之间。结论：该方法实验操作简单，专属性、准确度、重复性及稳定性良好，能够初步确定巴戟天质量标准，具有一定参考价值。

【关键词】高效液相色谱法；蒸发光散射检测器；巴戟天；含量测定；耐斯糖

ContentDeterminationMethodStudyontheMorindaOfficinalisRadixBasedonHPLC-ELSD

[Abstract]Objective:ThroughthemethodofHPLC-ELSDtodeterminethequalitycontrolstandardandestablishafast,simpleandaccuratemethod.Methods:Contentdeterminationwasperformedusinghigh-performanceliquidchromatography–evaporativelightscatteringdetectoronNystoseandSucroseofMorindaofficinalisRadix.Column:ECOSIL120-5-C18AQcolumn,mobilephase:methanolwatermixedsolution(3:97),flowrate:1.0mL/min,columntemperature:30℃,injectionvolume:10μL.Results:Welllinearintherangeof19.9742μg/mLto59.9226μg/mL,Thelinearequationis:y=41.873x-576.2,R2=0.9954,PrecisionandreproducibleRSDvalueswerelessthan2%.Thesamplesolutionhasgoodstabilityin12hours.Thesucrosewaslinearlycorrelatedwellintheconcentrationrangeof18.0788μg/mLto54.2361μg/mL,Thelinearequationis:y=51.166x-606.28,R2=0.9965,PrecisionandreproducibleRSDvalueswerelessthan2%,Thesamplesolutionwasbasicallystablewithin12hours,Therecoveryrateofthenicesugaradditionisbetween97.15%and100.15%;Sucroserecoveryratesrangedbetween94.00%and98.85%.Conclusion:Thismethodissimpleinoperation,hasgoodspecificity,accuracy,repeatabilityandstability,andcanpreliminarilydeterminethequalitystandardofserd,andhascertainreferencevalue.

[Keywords]High-Performan