大气细颗粒物染毒致C57BL6和C3HHe品系小鼠基因表达的差异（职称论文资料）.docVIP

大气细颗粒物染毒致C57BL6和C3HHe品系小鼠基因表达的差异（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：大气细颗粒物染毒致C57BL6和C3HHe品系小鼠基因表达的差异 1 1 材料和方法 3 2 实验结果 5 3 讨论 5 文2：高铁饮食致C57BL6小鼠黑质DA多巴胺能神经元选择 6 1 材料和方法 8 2 结 果 9 3 讨 论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 12 正文 大气细颗粒物染毒致C57BL6和C3HHe品系小鼠基因表达的差异（职称论文资料） 文1：大气细颗粒物染毒致C57BL6和C3HHe品系小鼠基因表达的差异 Different Expression of Genes between C57BL/6 and C3H/He Mice Exposed to Fine ParticlesCAO Qiang1，ZHANG Shu2，JIANG Zhi-hai3，Dong Chen3，Jiang Rong-fang1，Song Wei-min1*（ of Public Health，Fudan Univeity，Shanghai 200032，China； International Airport Examination Quarantine Bureau，Shanghai 201202，China； Municipal Center for Diseases Control and Prevention，Shanghai 200336，China） Abstract： ［Objective］ To study weather there are different expression of genes between C57BL/6（B6） and C3H/He（C3）mice after exposure to fine particles. ［Methods］ By intratracheal itillation，C57BL/6 strain（B6）and C3H/He strain（C3）mice were exposed to once per day for two coecutive days. Twenty-four hou after the last exposure，the mice were killed and lungs were taken out and immeed into liquid nitrogen immediately. Using the methods of RT-PCR，the expression of genes was examined in both B6 and C3 mice. ［Results］ It was found that expression of the genes of Cxcl2，C4，Hc and Cp in B6 mice exposed to were significantly higher than that in C3 mice exposed to . The results of image analysis showed that the quantities of expression of genes in B6 exposed to were ±，±，± and ±，and in C3 were ±，±，± and ±，individually. But expression of the genes of Igh-6 and Cap1 in B6 exposed to were significantly lower than that in C3 mice exposed to . The quantities of expression of genes in B6 were ± and ±，and in C3 were ± and ±，individually. ［Conclusion］ After exposed to ，the expression of genes was different between C57BL/6 and C3H/He mice. This might be one of the reaso that different genetic background can lead to different respoe to . Key Words：；lung injury；oxidative str