尾悬吊模拟失重对大鼠肺组织细胞凋亡的影响及其干预（职称论文资料）.docVIP

尾悬吊模拟失重对大鼠肺组织细胞凋亡的影响及其干预（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：尾悬吊模拟失重对大鼠肺组织细胞凋亡的影响及其干预 1 1 材料和方法 3 2 结果 5 3 讨论 6 文2：原位肝移植缺血再灌注损伤对大鼠肾脏组织细胞凋亡的影响 8 1 材料与方法 8 2 结 果 9 3 讨 论 10 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 尾悬吊模拟失重对大鼠肺组织细胞凋亡的影响及其干预（职称论文资料） 文1：尾悬吊模拟失重对大鼠肺组织细胞凋亡的影响及其干预 Study of the Effect and Its Protection of Simulated Weightlessness on Cell Apoptosis in Pulmonary Tissues of Tail-suspended RatsWANG Jun-feng，LIU Chang-ting，HAO Cong-jun，WANG De-long（General Hospital of PLA，Beijing 100853，China） Abstract：［Objective］ To investigate the changes and its protection of cell apoptosis in pulmonary tissues of tail-suspended rats in simulated weightlessness condition. ［ Methods ］ Experiments were conducted on 50 Spragno-Dawley male rats. The rats were divided into 5 groups：. tail-suspended for 7 days group with its control group，tail-suspended for 21 days with its control group，and dexamethasone treatment at the 3rd day of tail-suspeion group. Tail-suspeion（-30°）was used as weightlessness simulation. The condition of cell apoptosis in pulmonary tissues was detected with in situ death detection，and the condition of BCL-2 and BAX mRNA in pulmonary tissues was detected by hybridization in situ. ［ Results ］ There were apoptotic cells in pulmonary tissues of rats in general. The level of cell apoptosis in pulmonary tissues of 7 d tail-suspended rats increased significantly（P ）. The level of BCL-2 mRNA and of BAX mRNA in pulmonary tissues of 7 d tail-suspended rats decreased and increased respectively with statistical significance（P ）. The level of cell apoptosis in pulmonary tissues after dexamethasone treatment decreased significantly than that in tail-suspeion group（P ）. ［ Conclusion ］ There were excessive apoptotic cells in pulmonary tissues of 7 d tail-suspended rats in simulated weightlessness condition and BCL-2 and BAX mRNA showed corresponding changes. Dexamethasone can be used to inhibit pulmonary tissues apoptosis in simu