地木耳人工培养最适光温条件及功能性成分Scytonemin的-食品科学.pdf

208 2011, Vol. 32, No. 09 食品科学 ※生物工程 Scytonemin 赵丽娟，上官旖旎，陈娟娟，严小军 * (应用海洋生物技术教育部重点实验室(宁波大学) ，浙江 宁波 315211) 以宁波产野生地木耳(Nostoc commune)为对象，研究地木耳人工培养的最适光温条件，并就光温条件以及 紫外辐射对地木耳抗紫外活性物质 Scytonemin 产出量的影响进行探讨。结果表明：地木耳人工培养的最适光温条 件是 25 ℃，光照强度25 μE/(m2 ·s) 。同光照条件下，地木耳对较低的温度适应性比对较高的温度适应性好，而 光照强度高低的影响差异并不明显。不同的光温条件对地木耳中 Scytonemin 产出量及其变化有显著影响：最适光 温条件下，人工培养的地木耳中 Scytonemin 产出量最低(约 150μg/g) ，增加光强而不是增加光照时间对Scytonemin 产出量累积有利，光强增加 1 倍，产出量可增加近 120%。适当的紫外照射能显著提高地木耳中Scytonemin 的 产出量。 地木耳；人工培养；温度；光照；scytonemin Optimum Temperature and Light Conditions for Culturing Nostoc commune and Their Effects on the Functional Compound Scytonemin ZHAO Li-juan ，SHANGGUAN Yi-ni，CHEN Juan-juan ，YAN Xiao-jun* (Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education, Ningbo 315211, China) Abstract ：Nostoc commune is not only one of the traditional edible fungi in China, but also a natural multi-functional product. Artificial culture may change its growth and the accumulation of functional compounds. In present study, we attempted to establish the optimal conditions of temperature and light for the artificial culture of a local strain of N. commune in Ningbo, and to probe the effects of temperature, light, and ultraviolet radiation on the production of the functional compound scytonemin. The results showed that the optimal conditions of temperature and light were 25 ℃ and 25 μE/ (m2 ·s). At the same light intensity conditions, the growth of N. commune was faster at lower temperatures than at higher ones, whereas no significant changes occurred upon the light intensity. The temperature and light affected the production of scytonem