黄芩愈伤组织培养及其次级代谢物合成调控的研究-植物学专业毕业论文.docxVIP

————鉴堇窒堑篓篓登耋墨整姿鍪堡登垄窒塞鎏鎏墼鳖墓．一—— ————鉴堇窒堑篓篓登耋墨整姿鍪堡登垄窒塞鎏鎏墼鳖墓．一—— 愈经缌织，生物魁达28。7 gL～，总黄酮熬含鬟为354．6 mg,g1 t其中黄芩 营麓禽肇鸯167。4 mg-菩2，产慧达婺4．8菪L一，分糕怒瓣爨装5．54镶鸳t8+2 倍。 就舞，本论文迸研究-r=种前俸(己酸铺、L～笨溺氨酸帮貉氨酸)和三 释落警予臻l酸镣铵、磷酸镶霸承杨黢瓣黄芩塞魏缝织生长鹣赞苓营舔繁 的彩晌，鞋0．5 mnl01．L-‘瓣乙酸锛和0．01 mmol·L4瓣礴漆辚铵惑渠最鲟， 通过二者的协同作用最终可以穗黄芩静的产遂隗对照键高161％，产量达 i。33 g-01。 关键词：黄芩；愈伤组织；越芩苷：前体物；诱导子 ——塑!!垫筌! ——塑!!垫筌! 一． ABSTl扒CT Seutetlaria baicalensis Georgi氍baiealensis)is an herbaceous perennial plant used as a medicinal plant，which roots have been used for cleaning away heat，moistening aridity,purging fire，detoxi黟ing toxicosis，and is also anti—abortion agent。With the in—depth study on medicinal functions，the market demand of S baicalensis greatly rises．But the wild resource was explosively exploited in the long time and is dying Out in most legions．The manual planting is also confronted with many problems such as the long Iife cycle，the low livability,the excessive residual pesticide and the main activity constituents with tow content．At present，plant cell culture is one of the most important ways to produce secondary metabolite many Chinese traditional and herbal drugs． In this dissertation，following the basic principles of biology,and the help of the multi—subject analysis，the author studied the regulation，biosynthesis and accumulation rules of baicalin in＆baicalensis callus．Its objective is not only to discuss the possibility of industrialization for S baicalensis but also to provide the theory base tbr the field planting。Furthermore，the important conclusion will be applied to improve the yield ofS．baicatensis by the farm planting and the farm mode wilt be optimized by our experiment。 In this dissertation papeL callus was induced by plant cell culture technology．The content of baicalin was determined by HPLC。Different carbon source，nitrogen source，growth regulating substance，KH2P04 and organic addition were added to media。Their effects on baicatin and flavonoid content of S baiealensis callus were studied．Three precursors fs